摘要
以棒状链霉菌(Streptomyces clavuligerus),B71为出发菌株,通过紫外线诱变育种,采用琼脂块法初筛和摇瓶发酵复筛,选育出2株甘油耐受性正向突变株S.clavuligerus B71—14和S.clavuligerus B71-49,在摇瓶条件下,其克拉维酸产量与出发菌株S.davuligerus B71相比,分别提高了90.6%和88.8%,并具有良好的遗传稳定性。
The original strain Streptomyces clavuligerus B71 was treated with UV light, and methods including an agar column solid fermentation model and the shake-flask model were used for primary and further selection. Finally, S. clavuligerus B71-14 and S. clavuligerus B71--49 with high glycerol-resistant were ob- tained and their clavulanic acid yield increased 90.6% and 88.8% compared with S. clavuligerus B71. This character can be inherited stably.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2007年第5期9-12,共4页
Food and Fermentation Industries
基金
天津市2004年自然科学重点基金(043802711)
关键词
棒状链霉菌
紫外诱变
耐甘油
克拉维酸
Streptomyces clavuligerus, ultraviolet mutagenesis, glycerol-resistant, clavulanic acid
