摘要
采用乙酸乙酯提取试样中的氯霉素,然后将乙酸乙酯蒸发至干,用正己烷净化,再用乙酸乙酯提取氯霉素并进无水硫酸钠脱水,经C-18小柱净化。硅烷化后用微池电子捕获器的气相色谱仪进行测定,外标法定量。结果表明,高效液相色谱法检测氯霉素是一种灵敏度较高,可靠性较强的方法,此法重复性好,假阳性少,可以进行定量鉴定。
Draw chloramphenicol of sample with second sour second ester, then evaporate sour second ester of second to doing, with own alkane purify, draw chloramphenicol and carry on anhydrous sulphuric acid sodium dehydrate with second sour second ester and then, purified by C-18 pillar. Determine with the gaseous phase chromatograph of the electron catching de- vice of little pool after the silicon alkanisation, mark legal quantity outside. Result indicate high-efficient liquid phase chromatogram law measure chloramphenicol one relatively high sensitivity, a kind of relatively strong of dependability method, this repetitiveness is good, false and positive and little, can carry on the ration to appraise.
出处
《农产品加工（下）》
2007年第6期88-90,共3页
Farm Products Processing
关键词
氯霉素
水产品
气相色谱
chloramphenicol
aquatic products
gaseous phase chromatogram
