摘要
从雌性骆驼输卵管、子宫、子宫颈、阴道组织中提取总RNA,根据已发表的骆驼β-防御素-1基因的cDNA序列设计合成引物,采用RT-PCR扩增出了骆驼β-防御素-1基因;将扩增产物克隆于pBlueselectT载体后进行了序列分析。以β-肌动蛋白(β-actin)基因作为内参,对扩增的β-防御素-1基因进行琼脂糖凝胶电泳后,应用凝胶成像分析系统,推断出了不同组织中β-防御素-1基因的表达量。结果,从雌性骆驼生殖各组织上皮均获得了203 bp的β-防御素-1基因的扩增片段,且β-防御素-1基因在雌性骆驼生殖道各组织内的表达量不同。结果表明,β-防御素-1在雌性骆驼生殖组织的先天免疫中起重要作用。
Total RNAs were extracted from fallopian tube epithelial tissue, uterus epithelial tissue, cervixepithelial tissue and vagina epithelial tissue of camel. Based on the known β-defensin-1 (caBD-1) cDNA sequence, a pair of primers was designed, and the camel β-defensin-1 gene was amplified by RT- PCR. The purified RT-PCR product was cloned into pBlueselect T vector and sequenced. Using the same PCR conditions, a β-actin gene was amplified as the reference gene. Electrophoretic analysis showed that the β-defensin 1 gene was expressed in different genital tract tissues of camels, and the level of expression of the gene was different in different tissues. It was concluded that β-defensin 1 gene played an important role in the innate immune system of genital tract of camels.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2007年第6期515-518,共4页
Chinese Veterinary Science
基金
国家自然科学基金资助项目(30460093)