摘要
目的应用化学致癌物3,2’-二甲基-4-氨基联苯(3,2-’dimethyl-4-aminobiphenyl,DMAB)建立hamsters鼠浸润性膀胱癌的动物模型,用于非乳头状侵袭性膀胱癌的研究。方法用DMAB和乙炔基雌二醇(ethinyl estradiol,EE)处理hamsters鼠后,观察膀胱癌的发生情况,同时通过常规病理学检测方法,评价了Hamsters鼠膀胱癌的组织病理学特征。结果20周到50周期间,雄性鼠DMAB+EE组膀胱癌的发生率为78%(14/18),DMAB组中为65%(11/17),雌性鼠DMAB+EE组膀胱癌的发生率为79%(11/14),DMAB组为80%(4/5)。大部分膀胱癌发生在膀胱颈部,所有肿瘤均为非乳头状浸润性移行细胞癌,部分为腺癌或鳞状上皮化生癌。结论膀胱癌的浸润程度与移行细胞癌的异型性有关,与腺细胞或鳞状细胞的分化程度无关。在无DMAB处理的动物中没有膀胱癌的发生。性别及EE对DMAB的诱发的浸润性膀胱癌无关。
Objective Histopathological characteristics of urinary bladder tumors induced in Syrian golden hamsters by 3,2＇- dimethyl-4- aminobiphenyl （DMAB） were analyzed.Methods DMAS and ethinyl estradiol （EE） were adnfinistered to lhantsters and the tumor incidence8 in treated animals were detected. Histopathology was used for estimating the feature of tumor pathology. Results Urinary bladder eareinomas were induced in 14 of 18 hamsters （78% ;0.89/animal） in the DMAB ＋ EE group and 11 of 17 （65% ;0.88/animal） in the DMAB alone group in males,and in 11 of14 （79% ;0.79/animal） in the DMAB ＋ EE group and4 of 5 （80% ;0.80/animal） in the DMAB alone group in females examined between weeks 20 and 50. All were non-papillary invasive transitional cell carcinomas partly demonstrating glandular and/or squamous differentiation, and most carcinomas developed in tile bladder neck. Condusion Degree of invasion was dearly correlated with degree of morphological atypism in the transitional cell carcinomas, but riot with squarnous or glandular differentiation. No sex difference or modifying effect of EE on DMAB urinary bladder careinogenesis was evident. No bladder carcinomas were observed in non-DMAB-treated animals.
出处
《中国实验诊断学》
2007年第6期722-724,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技发展计划项目(编号:200505141)*通讯作者
关键词
膀胱癌
浸润癌
3
2’-二甲基-4-氨基联苯
乙炔基雌二醇
Urinary bladder carcinogenesis
Invasive cancer
3,2＇ -Dimethyl-4-aminobiphenyl
Etlfinyl estradiol
Hamster
