摘要
对95份抗—HCVIgG阴性献血员采用逆转录聚合酶链反应法（PCR）检测丙型肝炎病毒RNA，结果8次中有6次其检测出17份阳性标本（17／95，17．9％），复查抗—HCVIgG仍为阴性。对其中8份阳性产物中高变区1的序列分析结果表明均为不同株HCV序列．排除了PCR污染的可能性。对其中2份阳性产物测定了全序列并与HCV各基因型代表株的相应序列比较，与HCVⅡ型相应序列的核苷酸同源性为77％～79％。而与HCVⅠ、Ⅲ、Ⅳ相应序列间的同源性为62％～69％，表明为HCVⅡ型序列。结果提示献血员抗—HCVIgG筛选不能完全排除HCV感染者，漏检不是由于HCV基因序列变异．而是检测方法本身缺陷所致。
Reverse transcription polymerase chain reaction was used to detect hepatitis C virus (HCV) RNA in 95 serum specimens of blood donors with anti-HCV IgG negative. Six out of 8 detections, HCV RNA was detected in 17 of 95 specimens (17. 9% ). These 17 HCV RNA positive specimens were detected once more for anti HCV IgG to confirm their negative detection. Sequencing data of hypervariable region from 8 of 17 positive PCR products showed that the amplificated sequences came from different HCV strains, and were not the false amplification because of PCR contamination. In these 8 PCR products, two nucleotide sequences were determined at fulllength, and showed higher homology of 77%~79% with the corresponding sequences of representative strain of HCV genotype Ⅱ than that of 62%~69% with the sequences of HCV genotypes Ⅰ, Ⅲ, Ⅳ, and were verified as HCV genotype Ⅱ sequences which are most popular among Chinese HCV infected cases. Our data demonstrated that anti-HCV IgG detection is not sensitive enough for HCV screening in blood donors and the unavailable detection for HCV infection by anti-HCV IgG assay is not due to HCV sequence mutations.
出处
《中国病毒学》
CSCD
1997年第1期38-42,共5页
Virologica Sinica
基金
国家自然科学基金!39470037
