摘要
目的了解低频振动对脊髓损伤(SCI)继发骨质疏松(OP)大鼠骨代谢和骨质量的影响。方法50只SD大鼠采用脊髓全切横断法在第10胸椎处横断脊髓制作完全性SCI模型,并随机分为SCI6周对照组(SCI6w)、SCI12周对照组(SCI12w)、振动6周组(Vi6w)、振动12周组(Vi12w)和SCI6-12周振动组(Vi6-12w),每组10只;振动组分别于SCI后第4天(Vi6w组、Vi12w组)、第7周(Vi6-12w组)开始接受振动干预,振动频率20Hz,加速度0.15g,10min/次,2次/d,6d/周,Vi6w组和Vi6-12w组共振动6周,Vi12w组振动12周。分别在振动结束1d后处死动物,进行骨代谢血生化指标、骨密度(BMD)、骨形态计量学和生物力学性能测试。结果Vi6w组大鼠血Ca和股骨近端BMD较对照组改善(P<0.05);Vi12w组第5腰椎最大载荷较对照组升高(P<0.05);Vi6-12w组大鼠骨代谢和骨质量未见明显变化。结论早期开始的低频振动干预可改善SCI继发OP,但存在部位差异;后期进行的振动干预不能改善SCI继发OP,但可防止其进展。
Objective To observe the effect of low frequency vibration （LFV） on bone metabolism, bone mass density （BMD）, bone histomorphometric and biomechanical characterization in rats with secondary osteoporosis （OP） after spinal cord injury （SCI）. Methods SCI model was established with 50 SD rats by transecting spinal cord completely at the level of tenth thoracic vertebra, and the animals were randomly divided into 5 groups.. SCI 6-week control group, SCI 12-week control group, vibration 6-week （Vi 6w） group, vibration 12-week （Vi 12w） group and vibration 6-12 week （Vi 6-12w） group. All rats of vibration groups accepted LFV （frequency=20 Hz, accelerations0.15 g） with 10 minutes once, twice per day, and 6 days per week. The LFV was given from the forth day postoperation to the end of the sixth week in Vi 6w group, and to the end of twelfth week in Vi 12w group, while from the seventh week postoperation to the end of twelfth week in Vi 6-12w group. All animals were sacrificed when LFV test finishied. The serum alkaline phosphatase （ALP）, bone glaprotein （BGP） and serum calcium （Ca^2＋ ）/BMD of femur and the fifth lumbar vertebra （Ls）/bone histomorphometric characterization of tibia/bone biomechanical characterization of femur and Ls were measured. Results Compared with control groups, the serum Ca^2＋ and BMD of promixal femur improved significantly in rats of Vi 6w group （P 〈0.05）, while the maximal load of L5 increased significantly in rats of Vi 12 group （ P 〈0.05）. There were no changes in rats of Vi 6-12w group （P 〈0.05）. Conclusion The early treatment of LFV may improve the secondary OP of rats with SCI, but the effect is different in different sites of rats body. The late treatment of LFV can＇t improve the secondary OP, but may keep rats with SCI from deteriorating.
出处
《中国康复理论与实践》
CSCD
2007年第6期504-507,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
中国康复研究中心资助
关键词
脊髓损伤
骨质疏松
低频振动
骨代谢
骨质量
spinal cord injury
osteoporosis
low frequency vibration
bone metabolism
bone mass
