摘要
目的探索通过腹腔注射不同剂量的细菌内毒素标准品(LPS),建立内毒素诱导一氧化氮(NO)升高的小鼠模型。方法50只小鼠,雌雄各半,分为5组,每组10只,分别腹腔注射生理盐水或不同浓度的内毒素标准品(LPS)溶液:Ⅰ组:每只小鼠注射0.2ml0.9%生理盐水;Ⅱ组:每只小鼠给予LPS2.5mg/kg;Ⅲ组:每只小鼠给予LPS5mg/kg;Ⅳ组:每只小鼠给予LPS10mg/kg;Ⅴ组:每只小鼠给予LPS5mg/(kg·d),共给药3d;观察给药后各组小鼠血浆、肝组织、脑组织中NO浓度并做统计学分析。结果Ⅰ-Ⅴ组小鼠血浆中NO浓度分别为(μmol/L):84.58±9.82;160.46±62.21;320.26±180.03;261.44±189.34;79.08±39.70;其中Ⅱ、Ⅲ、Ⅳ组与Ⅰ组有统计学差异;Ⅰ-Ⅴ组小鼠肝组织中NO浓度分别为(μmolo/gprot):16.39±1.06;15.25±0.09;20.41±2.06;22.12±1.59;20.29±1.59;其中Ⅰ、Ⅱ组与Ⅳ、Ⅴ组、与Ⅲ组间均有统计学差异;Ⅰ-Ⅴ组小鼠脑组织中NO浓度(x-±s)分别为(μmologprot-1):7.21±2.86;5.80±1.93;13.47±4.64;13.84±2.63;16.40±5.27;其中Ⅰ、Ⅱ组与Ⅲ、Ⅳ、Ⅴ组间有统计学差异。结论腹腔注射LPS标准品5mg/kg以上,可以建立内毒素诱导的NO升高的小鼠模型。
Objective To investigate the NO - increased mouse model induced by celiac injection of different dosage of LPS standard. Methods 50 mice , divided into 5 groups randomly, were celiac injected saline or different dosage of LPS. Group Ⅰ ： saline group, each mouse received 0.2ml saline （ 0.9% ） ; Group Ⅱ：low dosage of LPS group, each mouse received LPS of 2.5 mg/Kg ;Group Ⅲ： middle dosage of LPS group, each mouse received LPS 5mg/Kg ;Group Ⅳ ： high dosage of LPS group, each mouse received LPS 10 mg/kg ;Group V ： repeat injection of LPS group, each mouse received LPS 5mg/（Kgod） ,totally 3 days. The concentration of Nitrate Oxide in blood plasma, liver and brain were detected. Results The concentration of Nitrate Oxide （μmol/L）inblood plasma of group Ⅰ to Ⅴ are：84.58±9.82;160.46±62.21;320.26± 180.03;261.44± 189.34;79.08 ±39.70;in livers are ： 16. 39 ± 1.06;15.25 ±0.09;20.41 ±2.06;22.12 ± 1.59;20.29 ± 1.59;in brains are：7.21±2.86;5.80±1.93;13.47±4.64;13.84±2.63;16.40±5.27. Conclusion By celiac injection of LPS standard at a dosage no less than 5mgoKg - 1, a NO - increased mouse model could be made.
出处
《临床和实验医学杂志》
2007年第6期9-10,共2页
Journal of Clinical and Experimental Medicine
