摘要
探讨了二氢杨梅素对红细胞等材料氧化伤害的保护作用.在不同的二氢杨梅素浓度下,用过氧化氢或过氧化特丁烷(TBHP)对红细胞、血红蛋白和Hela细胞进行氧化伤害,以紫外-可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白,硫代巴比妥酸(TBA)法测定红细胞中的丙二醛(MDA),MTT法检测Hela细胞的活力.结果表明随着二氢杨梅素浓度的增加,红细胞溶血率、丙二醛和血红蛋白的生成量显著下降,而Hela细胞的活力则显著上升.研究证明二氢杨梅素有着良好的抗氧化效果和潜在的激发细胞活力的功效,具有深入研究和开发利用的价值.
The protective effect of dihydromyricetin in oxidative damage on erythrocyte-related materials were studied. While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide （H2O2 ） or tea-butyl hydroperoxide （TBHP） on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde （MDA） from erythrocyte was measured by thiobarbituric acid （TBA） colorimetry, the cellular viability of Hela cell was examined using MTT （3-（4, 5-dimethylthiazol-2- yl）- 2, 5-diphenyl tetrazolium bromide） assay. The hemolysis rate of erythrocyte, methemoglobin and MDA was found reduced while the concentration of dihydromyricetin were increased. However, cellular viability of Hela cell was found increased paralleling with the increased concentration of dihydromyricetin. Our data suggested strongly that dihydromyricetin has favorable anti-oxidation effect and stimulative potential of cellular vitality, is worthy of further research and of great value for exploration and utilization.
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2007年第2期99-102,112,共5页
Journal of Natural Science of Hunan Normal University
基金
广东省社会发展攻关项目(2003B31704)
广州市科技攻关重点资助项目(2003Z2-E0201)
