摘要
目的建立童康片中黄芪甲苷的含量测定方法。方法采用HPLC-ELSD法测定童康片中黄芪甲苷的含量;色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相∶水-乙腈(34∶66),流速:0.8 ml.min-1,ELSD参数:漂移管温度:95℃,N2流速:2.4 mol.min-1。结果平均加样回收率为100.6%,RSD为2.06%,回归方程:Y=1.6266X+4.0603,相关系数:r=0.9991。结论经过系统的方法学考察,所建立的测定方法具有操作简便、稳定、专属、可重复的特点,可用于童康片的质量控制。
Objective To establish the determination of astragaloside Ⅳin Tongkang Tablets. Methods HPLC-ELSD method was developed for the determination of astragaloside IV in Tongkang Tablets by using Kromasil C 18 （250 mm × 4, 6 mm, 5μm） column, a mixture of acetonitrile-H2O （34： 66） as mobile phase at the flow rate of O. 80 ml·min^-1. The parameters of drift tube and gas flow rate of the detector were set at 95℃and 2.4 L·min^-1 ,respectively. Results The average recovery was 100. 6% and the linear equation was Y= 1. 626 6X ＋ 4.060 3. The correlation coefficient was 0.999 1. ConcLusion The method is simple, stable, accurate and repeatable, and can be applied to quality control of Tongkang Tablets.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第6期1380-1381,共2页
Lishizhen Medicine and Materia Medica Research
