摘要
目的:观察丙酮酸乙酯(EP)对急性坏死性胰腺炎(ANP)肺损伤大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和肺组织高迁移率族蛋白B1(HMGB1)mRNA表达的影响,探讨丙酮酸乙酯治疗急性坏死性胰腺炎肺损伤的机制.方法:逆行性胰胆管注射50 g/L牛磺胆酸钠制作ANP模型.随机分成3组,对照组、ANP组和EP治疗组(40 mg/kg,每隔6h静脉注射一次).ELISA法检测血清TNF-α和IL-1β水平;半定量逆转录聚合酶链反应(RT-PCR)法检测肺组织HMGB1 mRNA表达,并观察血氧变化及肺组织的病理变化.结果:ANP组血清TNF-α和IL-1β水平在建模后6h达高峰,12h下降,在此两时点治疗组血清TNF-α和IL-1β水平明显低于ANP组(TNF-α:131.6±29.6 ng/L vs 196.3±16.3 ng/L,65.0±16.6 ng/L vs 90.2±20.1 ng/L,P<0.05;IL-1β:194.9±26.8 ng/L vs 223.0±34.8 ng/L,105.2±24.0 ng/L vs 130.4±23.0 ng/L,P<0.05).ANP组大鼠肺组织HMGB1 mRNA表达水平在ANP后12h明显升高,至24h仍维持在高水平.治疗组肺组织HMGB1 mRNA表达水平在各时间点均明显低于ANP组(0.68±0.11 vs 0.88±0.11,0.81±0.11 vs 1.04±0.10,1.08±0.08 vs 1.33±0.15,P<0.05),且同期肺损伤比ANP组轻.治疗组PaO_2均明显高于ANP组.结论:丙酮酸乙酯能显著抑制TNF-α、IL-1β和HMGB1等早晚期炎症因子,改善低氧血症,对ANP肺损伤有明显保护作用.
AIM： To investigate the effects of ethyl pyruvate （EP） on the levels of tumor necrosis factor-α （TNF-α, interleukin-1β （IL-1β） in serum and mRNA expression of high mobility group box 1 （HMGB1） in lung tissues, and explore the mechanisms of ethyl pyruvate in protecting the lungs against acute injury induced by acute necrotizing pancreatitis （ANP）.
METHODS： ANP model was induced by retrograde injection of 50 g/L sodium taurocholate into pancreatic duct. Animals were divided randomly into 3 groups： control group, ANP group, and EP treatment group. EP solution was administered intravenously every 6 hours （40 mg/kg once）. The serum levels of TNF-α and IL-1β were measured by enzyme-linked irnmunosorbent assay （ELISA）. The mRNA expression of HMGB1 in lung tissues was detected by reverse transcription-polymerase chain reaction （RT-PCR）. The partial pressure of blood oxygen （PaO2） was measured, and histological examination of the lungs was also performed.
RESULTS： The serum levels of TNF-α and IL-6 were rapidly increased after ANP model was induced, and reached peak at the 6^th hour, but they were decreased at the 12^th hour. In comparison with those in ANP group, the serum levels of TNF-α and IL-1β were markedly lowered in EP group 6 and 12 hours after ANP model was induced （TNF-α 131.6 ± 29.6 ng/L vs 196.3 ± 16.3 ng/L, 65.0 ± 16.6 ng/L vs 90.2 ± 20.1 ng/L, P 〈 0.05; IL-1β; 194.9 ± 26.8 ng/L vs 223.0 ± 34.8 ng/L, 105.2 ± 24.0 ng/L vs 130.4 ± 23.0 ng/L, P 〈 0.05）. The mRNA expression of HMGB1 in lung tissues was increased significantly at the 12^th hour and maintained to the 24^th hour after ANP model was induced, whereas in EP group, HMGB1 mRNA expression was significantly lower than that in ANP group at each time point （0.68 ± 0.11 vs 0.88 ± 0.11, 0.81 ± 0.11 vs 1.04 ± 0.10, 1.08 ± 0.08 vs 1.33 ± 0.15, all P 〈 0.05）. The injury of lung tissues in EP group was milder than that in ANP group, and the value of PaO2 was also obviously higher i
出处
《世界华人消化杂志》
CAS
北大核心
2007年第12期1364-1369,共6页
World Chinese Journal of Digestology
关键词
高迁移率族蛋白-1
急性坏死性胰腺炎
急性肺损伤
丙酮酸乙酯
酶联免疫吸附试验
逆转录-聚合酶联反应
High mobility group box 1
Acute necrotizing pancreatitis
Acute lung injury
Ethyl pyruvate
Enzyme-linked immunosorbent assay
Reverse transcription-polymerase chain reaction
