摘要
目的:观察β-榄香烯对四氯化碳肝纤维化大鼠TGF-β_1、α-SMA、Col-Ⅰ表达的影响方法:采用CCl_4皮下注射诱导Wistar♂大鼠肝纤维化模型,用β-榄香烯0.1 mL/100g剂量每天腹腔注射8 wk后,用苏木精-伊红染色(HE)和胶原纤维(Masson)染色观察大鼠肝脏病理变化,酶动力法检测肝功能.SP免疫组化法检测肝组织中α-肌动蛋白(α-SMA)、转化生长因子β_1(TGF-β_1)、Ⅰ型胶原(Col-Ⅰ)表达的变化,样本碱水解法检测肝组织中羟脯氨酸(HYP)的含量.结果:8 wk后,正常组、模型组、对照组及治疗组肝组织胶原纤维面积百分比分别为1.22%±0.24%,7.47%±0.81%,5.57%±0.78%,4.33%±0.48%,治疗组与模型组、对照组相比均有显著差异(P<0.01),并且治疗组肝组织纤维化程度分级较模型组逐渐好转,胶原纤维所占面积显著缩小;在肝组织中测得的Col-Ⅰ阳性面积比分别为3.022%±0.553%,9.998%±1.431%,7.554%±0.914%,4.587%±1.008%,治疗组与模型组、对照组相比均有显著差异(P<0.01).α-SMA和TGF-β_1在治疗组和模型组肝组织中的表达也有显著差异(3.172%±0.542% vs 5.605%±1.315%,P<0.01;2.868%±0.554% vs 5.653%±0.9%,P<0.01).结论:β-榄香烯对四氯化碳肝纤维化大鼠具有拮抗作用,主要是通过抑制肝星状细胞激活,降低TGF-β_1,α-SMA在肝组织中的表达,减少细胞外基质在肝脏中的沉积,从而延缓肝纤维化的进程.
To study the effect of zedoary rhizome extract （β-elemene） on the expression of transforming growth factor-β（TGF-β1）, m-smooth muscle actin （α-SMA） and type Ⅰ collagen- Ⅰ （Col- Ⅰ ） in rats with hepatic fibrosis.
METHODS： The.experimental model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride （CCl4） in Wistar male rats. β-elemene was intraperitonealy administered into the rats for 8 weeks （0.1 mL/100 g body weight per day）. The samples were stained with hematoxylin and eosin for histopathological examination. Masson staining was used to observe the liver fibrosis of rats. Liver functions were measured by enzymatic kinetic analysis. The levels of α-SMA, TGF-β1 and Col-Ⅰ expression in liver tissues were measured by SP immunohistochemistry method. The content of hydroxyproline in liver tissues was tested by specimen alkaline hydrolysis.
RESULTS： After eight weeks of treatment, the area percentages of collagen fiber in normal, model, control and treatment groups were 1.22% ± 0.24%, 7.47% ±0.81%, 5.57% ±0.78% and 4.33% ± 0.48%, respectively. The percentage was significantly lower in treatment group than that in model and control group （both P 〈 0.01）, and the histological remission and collagen fiber diminishment were also better in treatment group. The expression of Col- Ⅰ in normal, model, control and treatment groups were 3.022% ± 0.553%, 9.998% ±1.431%, 7.554% ±0.914% and 4.587% ±1.008 %, respectively, and it was also significantly lower in treatment group than that in model and control group （P 〈 0.01）. The levels of α-SMA and TGF-β1 expression were significantly different between treatment group and model group （3.172% ± 0.542% vs 5.605% ± 1.315%, P 〈 0.01; 2.868% ± 0.554% vs 5.653% ± 0.9%, P 〈 0.01）.
CONCLUSION：β-elemene can reverse the pathologic progression of CCl4 induced liver fibrosis by inhibiting the activation of hepatic stellate cells, down-regulating the expression of α-SMA, TGF-β1 an
出处
《世界华人消化杂志》
CAS
北大核心
2007年第12期1324-1330,共7页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.30500658~~
