摘要
目的探讨骨髓源性神经干细胞自体移植对癫大鼠海马的修复作用。方法雄性SD大鼠随机分为正常对照组、移植组和非移植组。无菌条件下分离大鼠骨髓基质细胞,在特定条件下培养、诱导其分化为神经干细胞;对移植组和非移植组大鼠建立颞叶癫模型,将诱导分化的神经干细胞自体移植至移植组大鼠右侧海马内,观察移植后1周、2周、4周、8周和16周模型鼠海马的形态学变化。结果移植组与非移植组海马CA3区锥体细胞数各时间点显著少于正常对照组(均P<0.01);与非移植组比较,移植组海马CA3区锥体细胞数于移植后第2～16周明显增多(均P<0.01);移植组各时间段之间差异有统计学意义(均P<0.01)。与正常对照组相比,移植组和非移植组海马损伤侧的Timm染色评分显著增高(均P<0.01);但移植组移植2周后各时间点评分显著低于非移植组(均P<0.01);非移植组随制模时间延长评分持续升高。MRI检查显示在神经干细胞移植后1周和2周时低信号改变区比较局限,此后低信号影随着时间的推移逐渐增大。结论骨髓源性神经干细胞自体移植至癫大鼠后能够在海马中生存并迁移,具有减轻海马CA3区锥体细胞缺失、抑制海人酸引起的苔状纤维发芽的修复作用。
Objective To explore the repaired effects of autotransplantation of bone marrow-derived neural stem cells （BMSCs） on hippocampus of epilepsy rats. Methods Male SD rats were randomly divided into groups normal control （NC）, graft and non-graft . Under sterile condition, the BMSCs of rats were isolated, and under specific condition the BMSCs were cultured to induce and differentiate into neural stem cells（NSCs）. Then the models of temporal lobe epilepsy were established in groups graft and non-graft , and the NSCs were autotransplanted into the right hippocampus of rats of graft group. The morphological changes of the hippocampus were observed at 1,2 ,4,8, 16 weeks after the transplantation respectively. Results The number of hippocampal CA3 pyramid cells of groups non-graft and graft significantly decreased than that in NC group（ all P 〈0.01 ） ,and compared with non-graft group, there were significantly increased in 2 - 16 weeks after the transplantation in graft group （all P 〈 0.01 ）. There were significant differences among different phases in graft group （ all P 〈 0.01 ）. Compared with NC group, the Timm staining scores of the injured hippocampus in groups graft and non-graft significantly increased （ all P 〈 0. 01 ）. Compared with non-graft group, the scores of different phases in graft group decreased significantly since 2 weeks after the transplantation （all P 〈0.01 ）. The scores in non-graft group increased gradually with the time going on. MRI testing showed that low signal region was a little localized since 1 and 2 weeks after the transplantation, while it spread more widely with the time going on. Conclusions When the bone marrow-derived NSCs are transplanted into hippocampus of the epilepsy rats, they can survive and migrate. They may relieve CA3 pyramid cells loss and inhibit mossy fibers sprouting leaded by kainic acid for repaired effect.
出处
《临床神经病学杂志》
CAS
北大核心
2007年第3期207-210,共4页
Journal of Clinical Neurology
基金
广东省科技厅重大科技项目[粤财企(2001)367]
关键词
骨髓基质细胞
神经干细胞
移植
海马
bone marrow stromal cells
neural stem cells
transplantation
hippocampus
