摘要
目的：探索内皮素－１（ＥＴ－１）及前列环素（ＰＧＩ２）在内毒素血症所致肝损害中的作用。方法：选用Ｗｉｓｔａｒ大鼠１２０只，分为对照组、内毒素组、内毒素＋ＥＴ－１抗体组、内毒素＋前列环素组。观察了伤后３、６、９、１２和２４ｈ血浆中ＥＴ－１、谷丙转氨酶（ＧＰＴ）的含量；肝组织中ＥＴ－１、ＰＧＩ２、乳酸脱氢酶（ＬＤＨ）和丙二醛（ＭＤＡ）的含量。结果：内毒素血症时，血浆和肝组织中ＥＴ－１明显升高。血浆ＧＰＴ、肝组织中ＬＤＨ、ＭＤＡ均显著升高。肝组织ＥＴ－１与ＭＤＡ呈显著正相关。内皮素抗体部分拮抗内毒素所致肝损伤。结论：内毒素血症时ＥＴ－１对肝组织有一定损害作用。ＰＧＩ２通过舒张血管平滑肌对肝组织有一定保护作用。
Objective: To observe the effects of endothelin 1 (ET 1) and prostacyclin (PGI 2) on hepatic damage in endotoxemia. Methods: One hundred and twenty Wistar rats were randomized into the control, endotoxin (10 mg/kg), endotoxin plus PGI 2 and endotoxin plus ET 1 antibody (1∶20000, 2 ml/kg) groups and killed in the 3rd, 6th, 9th, 12th and 24h hour respectively. Plasma and hepatic ET 1 concentrations were determined. Plasma glutamic pyruvictransaminase enzyme (GPT), hepatic PGI 2, lactate dehydrogenate (LDH), and malondialdehyde (MDA) were also measured. Results: In endotoxemia, ET 1 contents in plasma and the liver were markedly increased and hepatic ET 1 concentration was positively correlated to hepatic MDA concentration. ET 1 antibody partially ameliorated hepatic damages in endotoxemia. Conclusion: ET 1 can ameliorate hepatic damages during endotoxemia and PGI 2 can protect the liver through its dilating effect on the vascular smooth muscle.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第1期35-38,共4页
Journal of Third Military Medical University
关键词
内毒素血症
内皮素
前列环素
肝疾病
endotoxemia
endothelin
prostacyclin
liver disease/physiopathology
