摘要
目的:探讨生长激素在体外对胆管癌QBC939细胞增殖的影响。方法:将胆管癌细胞随机分为实验组(GH组)和对照组(NS组),GH组按剂量和培养时间分为50μg/L2h(GH50-2h),50μg/L24h(GH50-24h),100μg/L2h(GH100-2h),100μg/L24h(GH100-24h)4个亚组,NS组按培养时间也分为NS-2h、NS-24h两个亚组,2h和24h后吸取上清用ELISA法检测类胰岛素生长因子1(IGF1)并细胞计数;胆管癌细胞用不同浓度GH(50μg/L,100μg/L)培养24h后固定,以流式细胞仪测定细胞周期;同时在不同浓度GH(50μg/L,100μg/L)干预的培养液中行细胞爬片后固定,用原位杂交的方法检测类胰岛素生长因子1受体mRNA(IGF1R mRNA)。结果:培养液中加入GH2h后QBC939细胞无明显增多(P>0.05),但24h后细胞数目明显增多并有统计学意义(P<0.05),24h流式细胞仪检测显示S期细胞百分(S%)比和细胞增殖指数(PI)也明显增加(P<0.05)。IGF1R mRNA在胆管癌中呈阳性表达,且GH可诱导细胞IGF1R mRNA表达增强。结论:GH在体外能促进胆管癌QBC939细胞的增殖和分化。
Objective： To explore the influence of human growth hormone on proliferation of bile duct carcinoma cell line（ QBC939 cell） in vitro. Methods： The QBC939 cells during exponential growth stage were harvested and divided into experimental group（GH） and control group（NS）. The experimental group was separated into four sub- groups（50μg/L 2h, 50μg/L24h,100μg/ L2h,100μg/ L24h） according to the dose of GH and culture time. The control group was also divided into two sub -groups（ NS -2h,NS -24h） by the culture time. After 2h,24h, insulin - like growth factorl （ IGF1 ） was detected by using ELISA. The QBC939 cells cultured for 24h with various GH concentration（50μg/L, 100μg/L） were made into single cell suspentions and samples underwent subsequent cell cycle evaluation. IGF1RmRNA was tested with the method of hybridization in vitro. Results： There was no statistical significance of the difference between group GH and group NS after 2 hours culture （ P 〉 0.05 ）. GH stimulated cell growth in vitro and also elevated percent S phase and proliferation index（PI） of cells after 24h＇ culture（ P 〈 0.05 ）. IGF1RmRNA was expressed in QBC939 in contrast to control, and the expression boosted up according to the dose of GH. Conclusion： GH stimulates QBC939 cells growth and proliferation in vitro.
出处
《现代肿瘤医学》
CAS
2007年第6期751-754,共4页
Journal of Modern Oncology
基金
江苏省卫生厅135基金资助项目(编号:135-08)
关键词
生长激素
胆管癌
细胞周期
growth hormone
bile duct carcinoma
cell cycle
