摘要
研究了利用嗜麦芽黄单胞菌BT-112(Xanthomonas maltophilia BT-112)游离细胞生物催化合成α-熊果苷,系统探讨了温度、对苯二酚浓度、对苯二酚与蔗糖摩尔比、反应时间、转速、细胞浓度、磷酸缓冲溶液pH值和浓度对反应转化率的影响。最佳反应条件为:反应温度为25℃,菌体对对苯二酚的最大耐受度为30mmol/L,蔗糖和对苯二酚的摩尔比为20∶1,反应时间为45h,摇床转速为160r/min,细胞浓度为85g/L,磷酸缓冲溶液浓度为25mmol/L、pH值为8.0。在此条件下α-熊果苷转化率高达86.7%(以对苯二酚计算)。
α-arbutin is biosynthesized by whole cell method with Xanthomona maltophilia BT-112. The conditions for cell biosynthesized α-arbutin are investigated as follows ： temperature, 25 ℃ ; concentration of hydroquinone, 30mmol/L; tool ratio of sucrose and hydroquinone, 20 ： 1 ; time course of α-arbutin biosynthesis, 45 hours; rotational speed, 160r/min; concentration of Xanthomona maltophilia BT-112, 85g/L; concentration of K2HPO4- KH2 PO4 buffer solution, 25mmol/L; pH of K2 HPO4-KH2 PO4 buffer solution, 8.0. Under the above optimal conditions, the maximum of molar conversion yield based on the amount of hydroquinone supplied reaches 86.7 %.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第3期417-420,共4页
Microbiology China
关键词
α-熊果苷
黄单胞菌
发酵
生物催化
α-Arbutin, Xanthomona maltophilia, Fermentation, Biocatalyst
