摘要
以磁性固定化纤维素酶的酶活力为响应值,应用响应面分析法(Response Surface Methodology,RSM)对制备磁性固定化纤维素酶的主要工艺参数(固定化时间、pH值、离子浓度和酶用量)进行了优化,并且采用二次回归方程拟合了4种因素和酶活力之间的数学模型,决定系数(R2)为0.9024,失拟性分析表明,无失拟因素存在.数学模型得出的最优工艺参数为:固定化时间11.98 h、pH值5.2、离子浓度0.066 moL.L-1和酶用量202.7 mL,最优条件下得到的最大酶活力为28.45744 I U.g-1.
The conditions such as immobilized time, pH value, buffer solution concentration and the amount of cellulase for preparing magnetic immobilized cellulase were optimized by means of the response surface methodology （RSM）, the activity of magnetic immobilized cellulase was chosen as the response value. And the mathematical regression model between different variables and the activity of magnetic immobilized cellulase was obtained using regression equation. Determination coefficient （R2） was 0. 902 4, lack of fit did not exist. The results demonstrated the optimum parameters of preparing magnetic immobilized cellulase were. immobilized time of 11.98 h, pH value of 5.2, buffer solution concentration of 0.066 mol·L^-1, the amount of cellulase used of 202.7 mL. Under the optimum parameters, the maximal activity of magnetic immobilized cellulase was 28. 457 44 IU· g^-1.
出处
《陕西科技大学学报（自然科学版）》
2007年第2期30-35,共6页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金青年基金(20306007)
广东省自然科学基金重点项目(04105934)
国家自然科学基金重点项目(20436020)
关键词
响应面分析
磁性固定化纤维素酶
酶活力
固定化
magnetic immobilized cellulase
enzyme activity
response surface methodology
