摘要
Degenerated primer was designed based on the amino acid conserved regions of the reported plant disease resistance gene which encode leucine-rich repeats (LRR). A resistance gene analog (RGA) named LRR1 was obtained from the RNA of TcLr35 carrying Lr35 gene conferring resistance against wheat leaf rust by reverse transcription-polymerase chain reaction (RT-PCR). The fragment had an open reading frame (ORF) and the nucleotide sequence containing 215 bp in length that coded 58 amino acids. 3′RACE was carried out with gene specific primers designed based on LRR1 and 3′universal primer provided in the kit. An amplified fragment of 567 bp in length which overlapped with the known LRR1 sequence by 184 bp was obtained. Finally, a fragment of 598 bp containing the sequence of LRR1 except the 5′cDNA end was obtained.
Degenerated primer was designed based on the amino acid conserved regions of the reported plant disease resistance gene which encode leucine-rich repeats （LRR）. A resistance gene analog （RGA） named LRR1 was obtained from the RNA of TcLr35 carrying Lr35 gene conferring resistance against wheat leaf rust by reverse transcription-polymerase chain reaction （RT-PCR）. The fragment had an open reading frame （ORF） and the nucleotide sequence containing 215 bp in length that coded 58 amino acids. 3＇RACE was carried out with gene specific primers designed based on LRR1 and 3 ＇universal primer provided in the kit. An amplified fragment of 567 bp in length which overlapped with the known LRR1 sequence by 184 bp was obtained. Finally, a fragment of 598 bp containing the sequence of LRR1 except the 5＇cDNA end was obtained.
出处
《植物病理学报》
CAS
CSCD
北大核心
2007年第3期317-320,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30170602)
河北农业大学博士基金
