摘要
传统的CTAB DNA提取法步骤多.较烦琐,DNA产率低,而且由于酚、单宁、色素、多糖很难完全去除,容易影响随机扩增多态、微卫星等标记工作的效率.采用SDS裂解法提取了针禾属植物幼嫩叶片的基因组DNA,所获得的DNA可用于PCR反应.并且在针禾属植物的研究中得到了较好的结果.
The traditional CTAB DNA extract method is troublesome with many steps and the gross Genomic DNA extracted by it is very low. High content of phenolic terpenoids, tannin, pigments, polysaccharide, etc. usually affect the activity of Taq and lead to the failure of PCR reaction. Genomic DNA of tender leaf is extracted in SDS method. The genomic DNA can be applied as template DNA in PCR reaction. The results suggested that SDS procedure was suitable for Stipagrosits genome DNA extraction.
出处
《广东教育学院学报》
2007年第3期64-68,共5页
Journal of Guangdong Education Institute
基金
国家重大基础研究前期研究专项(2004CCA02800)
教育部"春晖计划"项目(Z2004-2-65048)
