摘要
目的：探讨环氧合酶-2（COX-2）、血管内皮生长因子（VEGF）、前列腺素（PGs）在不同宫颈组织,尤其在CIN、宫颈癌组织的表达及其相关性。方法：选择正常宫颈、宫颈炎（宫颈糜烂）和宫颈瘤样病变组各20例,宫颈癌组织40例（Ⅰ期10例,Ⅱ期20例,Ⅲ-Ⅵ期共10例）。采用RT-PCR、免疫组化法分别检测COX-2mRNA、COX-2及VEGF蛋白在宫颈的正常、炎性、CIN组织及不同分期宫颈癌组织的表达;放射免疫RIA法分别测量各组患者血清中的PGs含量,并分析其与临床特征的关系。结果：1）COX-2 mRNA、COX-2和VEGF蛋白在正常宫颈组织无表达;在炎性及CIN组织有表达,两者之间差异无统计学意义（P〉0.05）;在宫颈癌组织中呈明显高表达,差异有统计学意义（P〈0.05）,而与临床分期、分级无关;腺癌与鳞癌间的表达差异无统计学意义（P〉0.05）;癌灶直径≥4 cm者,阳性表达率明显高于直径〈4 cm者（P〈0.05）。2）PGs含量,特别是前列腺素E2（PGE2）在正常宫颈组血清中存在,在炎性及CIN组含量增加,两者与正常组相比差异均有统计学意义（P〈0.05）,但两者之间差异无统计学意义（P〉0.05）,在宫颈癌组PGS含量明显高于炎性组,差异有统计学意义（P〈0.01）。结论：COX-2可能是早期参与促癌发生发展的因子之一,在宫颈癌早期COX-2主要通过PGS致癌;VEGF与COX-2的表达呈正相关,可能是COX-2促癌发生发展的又一分子机制,在宫颈癌中晚期可能主要通过调节PGs的水平上调VEGF表达促进宫颈癌的浸润和转移。
OBJECTIVE： To study the expression of cyclooxygenase-2 （COX-2）, vascular endothelial growth factor （VEGF） and prostaglandins （PGs） in cervical tissues of differential pathological types, especially in cervical intraepithelial neoplasia （CIN） and cervical carcinoma, and their possible correlation with carcinogenesis. METHODS： The tissue blocks and blood samples from 20 normal cervix women, 20 cervix inflammation patients, 20 CIN patients and 40 patients with cervical carcinoma respectively were included in this study. COX-2 mRNA was examined by the reverse transcripton-polymeras chain reaction （RT-PCR）. COX-2 and VEGF proteins were measured by immunohistochemistry using monoclonal antibodies to them. PGs （PGE2, TXB2, 6-keto-PGF1a） were detected by radioimmunoassay （RIA）. RESULTS： The overall positive expression of COX-2 and the quantity of PGs, especially PGE2 in inflammation, CIN and cervical carcinoma were higher and much higher than those in normal cervix （P〈0.05）, and there was a close relationship between COX-2 and PGs. The positive expression rate of VEGF in cervical carcinoma was higher than those in normal, inflammatory and CIN cervix respectively （P〈0.05）. VEGF protein was occasionally expressed in CIN cervix. There was no association among COX-2, VEGF and clinicopathological parameters in cervical carcinoma. The expression of COX-2 and VEGF in cases of tumor in diameter more than 4 cm was higher than those with smaller tumor. CONCLUSIONS： COX-2 probably is a gene involved early in carcinogenesis of cervical carcinoma by the increase of PGs, and accelerates the progress of tumor by the increase of PGs and VEGF. Therefore the expression of PGs may be a prognosis marker for clinical diagnosis.
出处
《中华肿瘤防治杂志》
CAS
2007年第11期843-846,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
甘肃省自然科学基金(ZS031-A25-061-E)
