摘要
本实验应用组织学方法检查了１２头围产期黑白花奶牛，以确定其脂肪肝的发生率，以Ｔ淋巴细胞转化率为指标测定受试牛分娩前后细胞免疫功能状态，应用放射免疫法测定受试牛分娩前后外周血浆中孕酮（Ｐ４）和前列腺素Ｆ２α（ＰＧＦ２α）的含量及其变化规律，从而探讨奶牛发生脂肪肝对免疫力和繁殖力的影响。实验结果表明：（１）８头黑白花奶牛在产后８～２１ｄ内发生脂肪肝，发病率为６６．６７％；（２）脂肪肝患牛在产后２～４周内，淋巴细胞转化率（淋转率）比正常对照组奶牛极显著降低（Ｐ＜０．０１），虽然在产前及产后１周差异不显著（Ｐ＞０．０５）。两组牛在产后１～４周的淋转率皆比产前１周极显著降低，患牛产后第２周淋转率比产前１周极显著降低（Ｐ＜０．０１），而产后第３、４周与第２周相比又有显著回升（Ｐ＜０．０５）；（３）脂肪肝患牛产后第一次出现孕酮值明显升高的平均时间比正常牛显著延迟（Ｐ＜０．０５）；
The effect of fatty liver on immunity and fertility was studied in a total of 12 Friesian cows Fatty liver was assessed by liver biopsy Cellular immune function was tested using PHA induced lymphocyte blastogenic responses,meantime the concentrations of peripheral P 4 and PGF 2α were determined by radio immunoassay (RIA) The experiment showed:(1)8 Friesian cows (66 67%)were diagnosed as having fatty liver 8～21 days after calving (2)The lymphocyte blastogenesis was decreased more markedly in cows with fatty liver than that in normal cows 2～4 weeks after calving (p<0 01),but it was not different between normal cows and cows with fatty liver 1 week before and after calving,it just declined slightly in cows with fatty liver Lymphocyte blastogenesis was decreased more markedly 1～4 weeks after calving than that 1 week before calving in the two groups,it was markedly decreased 2 week after calving compared with that of 1 week before calving,but was increased more significantly 3 to 4 weeks after calving than that 2 weeks after calving in cows with fatty liver (p<0 05) (3)After calving,the first rise of peripheral P 4 concentration for cows with fatty liver was significantly later than that for the normal cows (p<0 05) (4)Peripheral PGF 2α concentration during the periods of 1～29 days after calving was not different between the two groups
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第2期115-119,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
脂肪肝
围产期
乳牛
繁殖力
孕酮
前列腺素
Fatty liver,Periparturient dairy cows,Fertility,Lymphocyte transformation function,Progesterone(P 4),Prostaglandin 2α (PGF 2α )
