摘要
以已知核苷为标样,并以冬虫夏草生药作对比,利用反相高效液相色谱法对古尼拟青霉小孢变种RCEF0864菌丝体和发酵液中核苷类化学成分进行分析,结果表明古尼拟青霉小孢变种RCEF0864菌丝体中主要含有胞苷、尿苷、鸟苷、肌苷、环磷腺苷和腺苷等6种,其中腺苷和尿苷的含量均为冬虫夏草生药的3倍左右,肌苷和鸟苷的含量则分别是冬虫夏草生药的7倍和10倍左右,尿苷的含量也高于对照品冬虫夏草生药。古尼拟青霉小孢变种RCEF0864发酵液中主要含有尿苷、胞苷、胸苷、肌苷、环磷腺苷和腺苷等6种核苷类物质,其中,腺苷和胞苷的含量均高于对照品冬虫夏草生药。
Composition of nucleosides in Cordyceps sinensis and the fermentation broth and mycelia of Paecilomyces gunnii var. minor RCEF0864 were determined by high performance liquid chromatography （HPLC）. The results showed that nucleosides in the mycelia of RCEF0864 contain mainly adenosine, uridine, guanosine, inosine, cytosine, adenosine and cyclophosphate while the fermentation broth of RCEF0864 contains mainly uridine, thymine, inosine, cytosine, adenosine, adenosine cyclophosphate. The results showed that the contents of the nucleosides in the fermentation broth and mycelial of RCEF0864 are many times more than those in Cordyceps sinensis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第6期184-188,共5页
Food Science
基金
国家自然科学基金项目(30570189)
安徽省自然科学基金项目(00042425)
关键词
古尼拟青霉小孢变种
菌丝体
发酵液
核苷
Paecilomyces gunnii var. minor
mycelia
fermentation broth
nucleosides
