摘要
本研究建立了以新疆本地胡麻油脚为原料的卵磷脂提取工艺及双波长薄层扫描法测定胡麻卵磷脂中磷脂酰胆碱含量的分析方法。提取工艺为水化油脚真空浓缩脱水,丙酮脱油得粗磷脂;粗磷脂用超临界流体二氧化碳萃取纯化得磷脂;乙醇提取后最终得到以磷脂酰胆碱为主的卵磷脂。含量测定结果表明胡麻卵磷脂中磷脂酰胆碱的含量为62.4%,平均回收率为99.6%,RSD=3.42%。方法经济实用、结果可靠。
The extraction technology of lecithin was proposed and the content of PC in lecithin was determined by dual wavelength thin-layer scanning chromatography. Coarse phospholipids was prepared first, then refined with supercritical fluid extraction and organic solvent extraction successively. The factors affecting the extraction rate were investigated through orthogonal test. The optimum extraction conditions were determined. The content of PC in lecithin is 62.4%, with the average recovery as 99.6% and the RSD as 3.42%. This method is economical, practical and reliable.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第6期135-137,共3页
Food Science
基金
新疆维吾尔自治区高校科研计划立项项目(XJEDU2006S29)
新疆师范大学2005年校基金资助项目
关键词
胡麻卵磷脂
提取工艺
含量测定
flax lecithin
extraction technology
content determination
