摘要
以壳聚糖微球为载体构建了固定化β-葡萄糖苷酶,研究了固定化和游离β-葡萄糖苷酶的酶学性质。结果表明:固定化和游离β-葡萄糖苷酶的最适pH均为4.5;固定化β-葡萄糖苷酶的最适温度为60℃,比游离酶低5℃;固定化β-葡萄糖苷酶的pH稳定性和贮存稳定性明显高于游离酶,但热稳定性略低于游离酶;固定化和游离β-葡萄糖苷酶的表观米氏常数和米氏常数分别为0.52 mmol/L和2.4 mmol/L;固定化β-葡萄糖苷酶重复分批酶解10 g/L的纤维二糖,其操作半衰期为31 d左右。
The enzymology characteristics of immobilized and free β-glucosidase were investigated. The optimal pH of immobilized and free β-glucosidase was 4.5. The optimal temperature of immobilized β-glucosidase was 60℃, which was 5℃ lower than that of free β-glucosidase. Its stabilities of pH and storing were obviously higher than that of free β-glucosidase, but its thermal stability was a little lower than that of free β-glucosidase. The apparent Km and Km of immobilized and free β-glucosidase were 0. 52 mmol/L and 2.4 mmol/L, respectively. The immobilized β-glucosidase was used to hydrolyze 10 g/L cellobiose solution re- peatedly, its half-life time was about 31days.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第3期29-33,共5页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(30471361)
江苏省高技术研究计划(BG2005327)
江苏省高校自然科学重大基础研究项目(06KJA22015)
