摘要
目的:观察电针对单纯性肥胖大鼠下丘脑瘦素和神经肽Y表达的影响,探索电针减肥的机制。方法:实验于2005-12/2006-06在中南大学湘雅医院中西医结合研究所实验室完成。①取1月龄刚断乳SD雄性大鼠,随机取6只饲以普通饲料为正常对照组,其他大鼠饲以高脂饲料,喂养3个月后,选择体质量超过正常对照组20%的单纯性肥胖大鼠12只,随机分为模型组和电针组2组,每组6只。②电针组大鼠电针双侧足三里、天枢、三阴交穴,采用疏密波,电流强度0.3～0.6mA,留针20min,1次/d,共20次;其他2组不电针。实验期间均饲以普通饲料。③观察实验大鼠体质量、体长、Lee's指数及体脂,采用Western-blot技术检测下丘脑组织中瘦素、神经肽Y表达的变化。结果:18只大鼠进入结果分析。①模型组大鼠体质量和Lee’s指数高于正常对照组[(451.8±14.8),(323.6±6.8)g;324.25±1.4,305.14±1.5;P均<0.01];电针组电针后体质量和Lee’s指数低于电针前[(372.2±20.4),(454.7±19.7)g;307.71±1.5,323.56±1.6;P均<0.01]。②模型组大鼠心包、肾周和附睾脂肪量均高于正常对照组(P<0.01);电针组电针后心包、肾周和附睾脂肪量低于电针前(P<0.01)。③模型组下丘脑组织中瘦素蛋白表达低于正常对照组(0.62±0.11,0.88±0.15,P<0.01),电针组高于模型组(0.85±0.13,P<0.01);模型组下丘脑组织中神经肽蛋白表达高于正常对照组(2.42±0.27,1.75±0.24,P<0.01),电针组高于模型组(1.87±0.21,P<0.01)。结论:电针有良好的减肥效果,其作用可能与电针增强下丘脑组织瘦素蛋白的表达、同时抑制下丘脑组织中的神经肽Y蛋白表达有关。
AIM： To explore the effect of acupuncture on leptin of hypothalamus and the expression of neuropeptide Y in rats with simple obesity, and explore molecular mechanisms of acupuncture therapy. METHODS： The experiment was conducted at the Laboratory of Integrative Traditional and Western Medicine Institute. Xiangya Hospital Affiliated to Central South University from December 2005 to June 2006. （1） Six male SD rats of one month old were randomly selected as the normal control group, which were fed with normal stuff, while others were fed with high-fat diet. Three months later, 12 rats with the body mass 20% greater than those in the normal control group were randomly divided into 2 groups with 6 rats in each group： The model group and electroacupuncture group. （2） Rats in electroacupuncture group were needled by electro-acupuncture at bilateral Zusanli. Tianshu and Sanyinjiao acupoints with disperse-dense wave at the current intensity of 0.3-0.6 mA with 20-minute retention once a day for 20 times. Rats in other 2 groups were not interfered with anything and fed with normal stuff in the experiment. （3） The body mass, body length, Lee＇s index and body fat of experimental animals were observed. The changes in leptin and neropeptide Y expression in the tissues of hypothalamus were detected by Western-blot methods. RESULTS： A total of 18 rats were involved in the analysis of results. （1） The body mass and Lee＇s index of rats in the model group were higher than those in the model group [（451.8±14.8）, （323.6±6.8） g;324.25±1.4,305.14±1.5; all P 〈 0.01], while they were lower in electroacupuncture group after the electroacupuncture than those before electroacupuncture [（372.2±20.4）, （454.7±19.7） g;307.71±1.5,323.56±1.6, all P 〈 0.01]. （2） The fat mass in heart sac, renal periphery and epididymis in the model group was higher than normal control group （P 〈 0.01 ）, while it was lower in the electroacupuncture group after the electroacupuncture than before （P �
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第21期4151-4154,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
