摘要
目的:建立一个稳定、损伤程度适中的脊髓损伤大鼠模型的技术及方法学特征。方法:实验于2004-09/2005-01在承德医学院临床技能实验室完成。选取30只Wistar大鼠,体质量230～260g,纵行切开大鼠背部皮肤及皮下组织,剥离棘突旁肌肉,切除T7棘突及椎板,暴露脊髓硬膜。根据打击物(重10g)调节高度的不同将30只大鼠分为3mm高度组、5mm高度组、8mm高度组,每组10只。高度使重物自然下落,打击T7段脊髓,迅速移开打击重物及打击头,逐层缝合伤口,喂养8周后行病理学检查。选取通过脊髓最大径的切片在低倍镜下进行显微摄影,照片上侧量并计算空洞或瘢痕的最大直径与同水平脊髓直径的比例。结果:纳入大鼠30只,死亡5只,存活率83.3%;其中3mm高度组死亡1只,5mm高度组死亡1只,8mm高度组死亡3只。3mm高度组3例标本镜下可见损伤段脊髓中有不规则的空腔,6例标本于脊髓内形成不规则瘢痕,损伤部位瘢痕最大直径与同水平脊髓直径比例为(10.0±5.2)%;5mm高度组9例镜下均可见损伤段脊髓中有一个长圆形或不规则的空腔,损伤部位瘢痕最大直径与同水平脊髓直径比例为(46.0±3.8)%;8mm高度组存活的7例镜下均可见损伤段脊髓全部中断,形成瘢痕与硬膜外组织分界不清。结论:5mm高度组存活的大鼠的脊髓均产生位于脊髓中央部或近中央部的空洞,损伤的位置和程度较恒定,是较为理想的损伤模型。
AIM： To establish a stable, appropriately injured technique to rat model of spinal cord injury （SCI）. METHODS： The experiment was accomplished in the Laboratory of Clinical Technique in Chengde Medical College between September 2004 and January 2005. Totally 30 Wistar rats weighing 230-260 g were divided into A, B and C groups randomly. All the rats were cut open in dorsal skin and subcutaneous tissue to dissect the muscle surrounding spinous process. The spinal dura were revealed after excising T7 spinous process and vertebral plate. The heights of object which weight was 10 g were 3 mm at A group, 5 mm at B group, and 8 mm at C group. The T7 level of the rats were hit by the object which dropped without obstruction. Removing the object immediately and sewing up the wounds. All animals were sacrificed at the 8^th week following the impact injury. The pathologic changes of the sample were observed under the light microscopy. The percentage of the maximum diameter of cavities or scar with horizontal spinal diameter was calculated. RESULTS： All 30 rats were applied, 5 of them died, and the survival rate was 83.3%. At A group, a rat died; The irregular cavities or scars were respectively observed in 3 or 6 SCI models. At B group, a rat died; The oval or irregular cavities were observed in other survival 9 SCI models. At C group, 3 rats died; The injured spinal cord were apartal and the scars blended with epidural tissue in other survival 7 SCI models. The percentage of the maximum diameter of cavities or scar with horizontal spinal diameter was （10.0±5.2）% in A group, （46.0±3.8）% in B group. CONCLUSION： At B group, the cavities appear in central or sub-central spinal cord of survival SCI model, and the position and degree are steady, so it is the ideal SCI model.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第21期4144-4146,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
