摘要
目的:建立兔荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)分析方法并对其在实验动物科学中应用进行探讨。方法:本实验以5个品种(系)兔为实验材料,对DNA提取、预扩增和选择性扩增等步骤进行优化并对结果进行检测。结果:5个品种(系)兔的扩增片段数均在120条以上,各品种(系)特异性条带均能找出,5个品种(系)兔能据此进行区分。结论:fAFLP是一种以PCR为基础的DNA指纹技术,为兔的指纹分析提供了一种快速高效的方法,可用于兔的遗传质量检测。
Objective：To establish fluorescent amplification fragment length polymorphism analysis system for rabbits and discuss the applications in laboratory animal science. Methods：Through refined procedure such as DNA extraction, pre-amplification, and selective amplification, the genome of five rabbit strains were detected. Results：The special band of each strain could be found and strains could be distinguished. Conclusion：Fluorescent amplification fragment length polymorphism analysis system is based on PCR technology and high-efficiency, it could be used for genetic inspect of rabbits.
出处
《军医进修学院学报》
CAS
北大核心
2007年第3期199-200,共2页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金资助项目(30170680)
