摘要
目的:探讨人肺癌在化疗后细胞膜表面糖复合物的变化规律。为进一步判定肺癌化疗疗效提供新的理论依据。方法:选用60例人肺癌标本分成术前化疗组和术前未做化疗组,用免疫组化,检测桑橙凝集素(MPA)、花生凝集素(PNA)、蓖麻凝集素(RCA)和麦胚凝集素(WGA)与化疗组和非化疗组人肺癌细胞的亲和性。结果:在肺癌术前未行新辅助化疗(对照组)与行新辅助化疗(实验组)中,其癌细胞都保持着与MPA较弱的亲和性,两组之间无显著性差异(P>0.05)。凝集素PNA、RCA、WGA与癌细胞的亲和性在实验组和对照组有显著性差异(P<0.01)。在肺癌术前行新辅助化疗后,凝集素PNA、RCA、WGA与癌细胞的亲和性较对照组明显减弱。同时也说明,癌细胞表面的糖基Galβ1,3GalNAc,Galβ和GalNAc,NeuNAc(β1,4Glc-NAc)1,2和βNeuNAc在化疗后明显减少或丧失。结论:人肺癌在化疗后细胞膜表面糖复合物的变化规律,有助于肺癌化疗疗效的判定。
Objective： To investigate the post-chemotherapeutic change in glycoconjugates on the surface of human lung cancer cell membranes, in order to provide a new theoretical basis for further assessment of chemotherapeutic effect on lung cancer. Methods： A total of 60 patients with lung cancer were divided into two groups： group A with chemotherapy before surgery and group B without. Immunohistochemical staining was used for detection of the binding affinities between the cancer cells in groups A and B and various glycoconjugates including Maclura pomifera agglutinin （MPA）, Arachis hypogea agglutinin （PNA）, Ricinus communis agglutinin （RCA） and wheat germ agglutinin （WGA）. Resuits： A fairly faint affinity to MPA was shown in the lung cancer cells in group A and group B. There is no significant difference between the two （P〉0.05）. There was a significant difference when comparing the affinities between the glycoconjugates, i.e. the PNA, RCA and WGA, and the lung cancer cells in the two groups （P〈0.01）. In group A, the affinity between the agglutinins, i.e. PNA, RCA and WGA, and the cancer cells was markedly weakened, which demonstrated that Galβ1, 3GalNAc, Galβ, GalNAc, euNAc （β1,4Glc-NAc）1.2 and βNeuNAc, the glycons on the surface of the cancer cells, decreased sharply or disappeared after chemotherapy. Conclusion： A drastic change in the presence of various glycoconjugates on the lung cancer cell surface after chemotherapy provides a method for assessing the chemotherapeutic effect.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第11期629-631,共3页
Chinese Journal of Clinical Oncology
基金
大连市卫生局科学基金资助(编号:39470739)
关键词
肺肿瘤
化学治疗
凝集素
Lung cancer Chemotherapy Lectins Human
