摘要
用犬瘟热（ＣＤ）弱毒和自制的高免犬血清，建立了检测犬瘟热病毒（ＣＤＶ）抗体的中和（ＳＮ）试验、间接荧光抗体技术（ＩＦＡＴ）和间接免疫酶染色法。这３种方法对３８份ＣＤ弱毒疫苗免疫犬血清抗体效价的测定结果表明，当被检血清稀释度为１∶１００时，ＳＮ试验、ＩＦＡＴ和间接免疫酶染色法测定时的阳性血清数分别为２９，７和３１份，ＩＦＡＴ和间接免疫酶染色法测定结果相对于ＳＮ试验结果的符合率分别为４２．１％和９４．７％。同时发现，当ＳＮ试验测定的血清效价在１∶１００以上时，ＩＦＡＴ测定结果总是在１∶２５以上，间接免疫酶染色法测定结果总是在１∶１００以上。３种方法以各自初步确定的ＣＤＶ血清抗体效价完全保护值指标计算所测血清的完全保护率，ＳＮ试验为７６．３％，ＩＦＡＴ为７８．９％，间接免疫酶染色法为８１．６％。据此认为，ＩＦＡＴ和间接免疫酶染色法均可部分代替ＳＮ试验用于ＣＤ疫苗免疫效果的评价。
Thirty eight serum samples from dogs vaccinated with attenuated caine distemper virus (CDV) were detected by serum neutralization (SN) assay, indirect immunofluorescence antibody technique (IFAT) and immunohistochemical technique, respectively. It was found that the positive serum samples were 29 in SN assay, 7 in IFAT and 31 in immunohistochemical technique when the sera were detected at 1∶100 dilution. The statistical analysis indicated that there were 42.1% agreement between IFAT and SN assay, and 94.7% agreement between immunohistochemical technique and SN assay.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第1期26-29,共4页
Chinese Journal of Veterinary Science
基金
军队"八五"医药卫生基金重点课题
关键词
犬瘟热病毒
抗体
间接荧光抗体
间接免疫酶染色
canine distemper
antibody
serum neutralization assay
indirect immunofluorescence antibody technique
immunohistochemical technique
