摘要
从暴发类似传染性法氏囊病（ＩＢＤ）的江苏省某鸡场采集病鸡法氏囊组织制成组织悬液，接种鸡胚卵黄囊，部分鸡胚３～５ｄ死亡，部分鸡胚不死亡但有病变。用感染胚卵黄囊和绒尿膜混合物，接种鸡胚成纤维细胞（ＣＥＦ），盲传３代后，发现以合胞体为特征的细胞病变（ＣＰＥ）。感染细胞做超薄切片电镜观察，可见细胞浆内病毒粒子呈整齐的晶格状排列。用免疫沉淀法提取病毒抽提核酸，经ＳＤＳ－ＰＡＧＥ电泳，可见规律排列的１０条带，呈３－３－１－３排列。与禽呼肠孤病毒（ＡＲＶ）参考毒株Ｓ１１３３株的核酸谱带的数目和位置相同。血清学试验与ＡＲＶ呈阳性反应，与传染性法氏囊病病毒（ＩＢＤＶ）呈阴性反应。
A strain of avian reovirus (ARV) was isolated from bursa of chickens probably infected with infectious bursal disease. The virus could replicate in chicken embryo yolk sac, resulting in embryonic death in 3￣5 days. After the virus was adapted to the chicken embryo fibroblast (CEF) cell culture, some cytopathic effect (CPE) characterized by the formation of syncytia occurred. In ultrathin section, the virions arranged in crystal line and presented as inclusion bodies in the cytoplasma of CEF. The viral genome was composed of ten segments with the migration pattern of 3 3 1 3 being identical to those of strain S1133 of ARV as determined by polyacrylamide gel electrophoresis (PAGE).
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第1期18-20,共3页
Chinese Journal of Veterinary Science
