摘要
为建立一种快速、简便、灵敏分光光度法测定葡萄糖酸锌方法，在非离子型表面活性剂聚氧乙烯失水山梨醇油酸单脂（Tween-80）及聚氧乙烯异辛基苯基醚（TritonX-100）存在下，用水溶性试剂2-（5-硝基-2-吡啶偶氮）-5-[N-正丙基-N-（3-磺酸丙基）氨基]苯酚二钠（简称Nitro-PAPS）作显色剂直接光度法测定葡萄糖酸锌。结果表明，该法显色络合物最大吸收波长为580nm，本法线性范围为0～1μg／mL，表观摩尔吸光数为1.41×10^5L．mol^-1.cm^-1，回收率为98.5％～102.8％。该法具有操作快速、简便、结果灵敏可靠等优点，应用本法直接测定补锌口服液葡萄糖酸锌含量，结果满意。
To establish a simple, sensitive and stable new method of determination of zinc Gluconate, 2-（5-nitro-2-pyridylazo）-5-（N- propyl-N-sulfopropylamino） phenol was used as a coloring reagent to determine zinc gluconateby spectrophotometry in the presence of surfactants Tween-80 and Triton X-100. The maximum absorption of the complex compound was at 580 nm. The linearity of test was 0.1～1μg/mL. The molar absorption coefficient is 1.41 X l0^5L.mol^-1.cm^-1. The recoveries ranged from 98.5% to 102.8%. This method is simple, sensitive, rapid and accurate. The method could be used for the determination of zinc in zinc gluconate solution with satisfactory results.
出处
《广东微量元素科学》
CAS
2007年第4期46-49,共4页
Trace Elements Science
基金
浙江省卫生厅科研基金资助项目(2002A064)
