摘要
目的探讨急性热应激(HS)后大鼠中脑导水管周围灰质Fos蛋白的表达情况。方法将大鼠分别置于24℃、34℃、38.5℃或42℃,湿度为60%的恒温恒湿舱内1h,断头取脑,常规切片,用亲合素-生物素-过氧化物酶复合物法(ABC)进行抗Fos蛋白的免疫组织化学染色。结果对照组大鼠中脑导水管周围灰质内只发现很少数的Fos阳性细胞。置于34℃的大鼠中脑导水管周围灰质内Fos阳性细胞有所增加;38.5℃大鼠Fos阳性细胞明显增加,数量达到高峰;42℃大鼠Fos阳性细胞数明显减少。Fos阳性细胞主要分布于中脑导水管周围灰质的腹外侧区。结论中脑导水管周围灰质,尤其是腹外侧区内神经元可能参与了对热应激反应的调节。
To investigate the expression of Fos protein in rat periaqueductal gray following acute heat stress （I-IS） . Methods Rats were exposed in the cabin at different temperature （24℃, 34℃, 38.5℃ or 42℃ ） with 60% humidity for 1 h. After rats were perfnsed, the brains were removed. staining was used to detect the expression of Fos protein. Results In control rats, a few Fos-like inummoreactive （Fos-LI） nuclei were identified. After heat stimulation at 34℃, the number of Fos-LI nuclei increased. At 38.5℃, the number of Fos-LI nuclei increased abruptly and peaked. At 42℃, the number of Fos-LI nuclei decreased significantly. Fos-Ll nuclei mainly are located in the periaqueductal gray, especially in ventral lateral area. Conclusion The neurons in periaqueductal gray may be involved in modulating heat stress.
出处
《中华神经外科疾病研究杂志》
CAS
2007年第3期223-225,共3页
Chinese Journal of Neurosurgical Disease Research
基金
军队"十五"重点课题基金资助项目(01Z082)
第四军医大学基金资助项目(05ZXJM001)
