摘要
应用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术检测了猪和牛的扁桃体、淋巴结、脊髓、肌肉和蹄冠皮肤中的口蹄疫病毒（ＦＭＤＶ）。与接种乳鼠测毒法比较，该方法的灵敏度达０．１６ＬＤ５０～０．３２ＬＤ５０病毒量。检测人工感染后７～３５ｄ的猪组织样品７７份，２５份为阳性；接种乳鼠并盲传３代，乳鼠－间接血凝试验（ＩＨＡ）鉴定６份为阳性。检测人工感染后４～７ｄ的牛组织样品５２份，２０份为阳性；接种乳鼠－ＩＨＡ鉴定１２份为阳性。检测野外鲜肉样品４７份，１２份为阳性；任选其中８份样品接种细胞单层，盲传至第６～７代，先后有５份样品产生ＣＰＥ；其中６份样品同时接种乳鼠，盲传至第６～７代，３组乳鼠出现ＦＭＤＶ致病症状，ＩＨＡ鉴定为阳性。检测冻猪肉样品３７份，３份为阳性；冻牛肉样品９份、冻羊肉样品１０份。
A reverse transcription polymerase chain reaction (RT PCR) technique was used to detect the presence of foot and mouth disaese virus (FMDV) in animal (pig and cattel, tissues (tosil, lymph node, spinal cord, meat and skin of foot). The RT PCR was able to detect 0.16 LD 50 ～0.32 LD 50 , at least 6～12 times more sensitive than infectivite assay. Of 77 swine tissue samples collected on 7～35 days post infection, 25 positive results were detected by RT PCR; and 6 positive by virus isolation IHA. 20 of 52 bovine tissue samples were positive by RT PCR, and 12 positive by virus isolation IHA. 103 field samples have been examined and the results were reliable and satisfactory.
出处
《中国兽医科技》
CSCD
1997年第1期3-4,共2页
Chinese Journal of Veterinary Science and Technology
关键词
口蹄疫
病毒
聚合酶链反应
foot and mouth disease virus (FMDV) pig cattel reverse transcription polymerase chain reaction (RT PCR)
