摘要
在小干松抗旱地理种源选择的基础上,采用抗旱种源在吉林省半干旱区的多个典型立地进行了比较栽培试验研究。结果表明,小干松更适宜于在沙荒地上营造。通过多种造林方法比较,总结出小干松沙地造林实用技术。利用ISSR-PCR方法对抗旱种源进行了基因多态分析,依据扩增结果进行遗传距离分析,构建分子树状图,探讨了小干松抗旱种源间的遗传关系和利用ISSR分析进行种源鉴定的可行性。
Seven drought - resistance provenances were cnltivated in 3 typical locality of middle - western and semi - dry region of Jilin province China, based on provenance selection of lodgepole pine （ Pinus conorta ）, Its gene polymorphism were determined by using ISSR marker assays, It was indicated that lodgepole pine more appropriate grows in sand,planting technique of lodgepole pine was affirmed by compared with 4 planting methods. The gene polymorphism analysis of drought - resistance provenance was conducted by using ISSR - PCR method. According to DNA amplification,the genetic distances was determined and analyzed, the molecular dendrogram was constructed. The heredity relations among drought - resistance provenances and the feasibility of provenance identification by ISSR analysis were discussed in this paper.
出处
《吉林林业科技》
2007年第3期1-7,27,共8页
Journal of Jilin Forestry Science and Technology
