摘要
聚合酶链式反应-单链构象多态性(PCR-SSCP)技术是一种简便、灵敏的突变检测方法。随着该技术的不断发展和完善,其应用也越来越广泛。尤其是近年来该技术在昆虫学研究中涉及了:基因突变位点的检测、昆虫分类鉴定、多样性分析及连锁图谱的构建等多个领域。文章综述PCR-SSCP技术的发现、原理、在昆虫学研究中的应用及其优点和不足之处。
PCR-SSCP is a simple and sensitive method to screen mutations. This method is applied more and more widely along with its development and maturation. In recent years, PCR-SSCP is used in many fields of Entomology: screening gene mutation, identifying insects, studying the diversity of the genes, and constructing the genetic linkage map.This review includes the principle, new discovery, advantages and disadvantages of PCR-SSCP and finally the applications of the method in entomology is intrduced.
出处
《昆虫知识》
CAS
CSCD
北大核心
2007年第3期333-336,共4页
Entomological Knowledge
基金
黑龙江省森林重大病虫害高效安全持续控制技术项目(GB06B304)