摘要
采集成熟的大鳞大马哈鱼精子,在不同的激活介质、人工精浆(ASP)、pH和温度条件下进行活力观察。结果表明:用生理盐水(BSS)激活的精子活力高于用水激活(P<0.05),单尾鱼的精子活力高于多尾混精(P<0.05);在ASP A,B和C液中,用ASP C液保存精子的效果最好(P<0.05);保持大鳞大马哈鱼精子活力较强的适宜pH值为8.19-8.40,而活力最强时pH值为8.40;在4℃下保存的大鳞大马哈鱼精子活力最高;大鳞大马哈鱼受精率最高时的温度和pH值分别为4℃和8.40,这与大鳞大马哈鱼精子活力最强时的温度和pH条件高度相关。
The activation and motility of spermatozoa from Chinook salmon collected by dissection were studied under different reactivators, ASP, pH and temperature. The results were as follows： sperm motility in BSS is higher than that in water（ P 〈 0.05）. Sperm motility of one fish is better than that of mixed milt（P 〈0.05）. In ASP A, B and C, ASP C is the best to preserve spermatozoa（P 〈0.05）. The pH value to help preserve strong motility of the spermatozoa is pH 8.19 - 8.40, and spermatozoa motility is the highest under the conditions of pH 8.40. When temperature is 4 ℃, sperm motility is the highest. The optimum temperature and pH for the highest fertilization of Chinook salmon are also 4 and 8.4 respectively, consistent with the conditions making the vitality of the spermatozoa highest.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2007年第3期338-342,共5页
Journal of Jilin Agricultural University
基金
国家"948"项目(203104)
关键词
大鳞大马哈鱼
精子活力
激活剂
人工精浆
PH值
温度
Chinook salmon
sperm vitality
reactivator
artificial seminal plasma （ASP）
pH value
temperature
