摘要
目的 探讨尼可地尔后处理和缺血后处理对大鼠心肌缺血再灌注损伤的影响。方法 健康成年SD大鼠24只,建立Langendorff模型灌注离体心脏,随机分为3组,每组8只,对照组（IR组）：全心缺血40min,再灌注Krebs-Henseleit缓冲液（K-H液）60min;缺血后处理组（IPC组）：全心缺血40min,再灌注10s、缺血10s,反复6次后,再灌注K-H液58min;尼可地尔后处理组（NPC组）：全心缺血40min,灌注含20μmol/L尼可地尔的K-H液10min,然后再灌注K-H液50min。测定缺血前5min、再灌注30min及结束时冠脉流出液中碱性磷酸激酶同工酶（CK-MB）活性,再灌注结束即刻取左心室组织,测定超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、ATP含量,电镜观察心肌超微结构。结果 与IR组比较,IPC组和NPC组再灌注期CK-MB活性及MDA含量降低,SOD活性和ATP含量增高（P＜0．05）,IPC组和NPC组上述指标比较差异无统计学意义（P＞0．05）。IPC组和NPC组的心肌超微结构损伤程度轻。结论 尼可地尔后处理和缺血后处理均可以减轻大鼠心肌缺血再灌注损伤,其机制可能与减少氧自由基的生成、增强心肌抗氧化能力和减少细胞能量消耗有关。
Objective To evaluate and compare the cardio-protective effects of nicorandil and ischemic postconditioning against ischemia-reperfusion injury in rats. Methods Twenty-four SD rats of both sexes （200-250 g） were anesthetizes with intraperitoneal urethane. Their hearts were excised and perfused with oxygenated Krebs-Henseleit buffer （KHB） in a Langendorff apparatus. The isolated hearts were randomly divided into 3 groups： I/R group： the hearts were subjected to 40 min global ischemia followed by 60 min reperfusion; ischemic postconditioning group： the hearts underwent 6 cycles of alternate 10 s ischemia and 10 s reperfusion immediately after 40 min ischemia and before 58 min reperfusion and nicorandil postconditioning group： the hearts were reperfused immediately after 40 min ischemia with nicorandil 20 μmol/L in KHB for 10 min followed by 50 min reperfusion with plain KHB. CK-MB leakage into the coronary effluent was measured at 5 min before ischemia and 30 and 60 min of reperfusion. Myocardial SOD activity and MDA and ATP contents were determined and ultrastructure of myocardium was examined at the end of reperfusion. Results The CK-MB leakage into coronary effluent was significantly less during reperfusion in the 2 postconditioning groups than in I/R group. The myocardial MDA content was significantly lower whereas the myocardial ATP content and SOD activity were significantly higher in the 2 postconditioning groups than in I/R group, but there was no significant difference between the 2 postconditioning groups. Conclusion Nicorandil postconditioning is as effective as isehemic posteonditioning in the protection of myocardium against I/R injury in rats. Decrease in oxygen free radicals and increased antioxidant activity and decreased energy consumption of myocardial cells are involved in the mechanism.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2007年第4期309-312,共4页
Chinese Journal of Anesthesiology
基金
重庆医科大学创新基金（CX0513）
关键词
尼可地尔
心肌再灌注损伤
缺血后处理
Nicorandil
Myocardial reperfusion injury
Ischemic postconditioning
