摘要
研究了固定化微环境对南方红豆杉细胞生长和紫杉醇合成的影响,结果表明:固定化细胞停滞期缩短,细胞膜脂氧合酶活性和脂过氧化产物含量远远高于悬浮细胞,同时苯丙氨酸解氨酶活性显著增加,微环境溶液内紫杉醇含量与悬浮细胞有显著性差异.研究表明固定化微环境能促进红豆杉细胞紫杉醇含量增加但对细胞膜脂产生了过氧化.
Cell growth and taxol synthesis of Taxus chinensis var, mairei were investigated under the immobilization microenvironment, The results show that the stagnation period of immobilized-cell is shortened. Lipoxygenase activity and malondialdehyde content of immobilized-cell are significantly higher than those of suspended-cell, Both phenylalanine ammonia-lyase activity of immobilized-cell and extracelluar taxol content in the microenvironment show a marked increase. An immobilization microenvironment can promote taxol content and peroxidize the cell membrane lipid.
出处
《山东大学学报（理学版）》
CAS
CSCD
北大核心
2007年第1期64-68,共5页
Journal of Shandong University(Natural Science)
基金
国家自然科学基金重点基金资助项目(20236040)
关键词
固定化
微环境
南方红豆杉细胞
紫杉醇
immobilization
microenvironment
Taxus chinensis var, mairei cells
taxol
