摘要
在15、20、25和28℃条件下培养核盘菌菌核,再在20℃下诱导菌核萌发,结果将不同来源的50个菌株分成5类:①菌核易进行菌丝型萌发;②4种温度下形成的菌核均易进行子囊盘型萌发;③高温(25和28℃)下形成的菌核进行子囊盘萌发,而低温(15或20℃)下形成的菌核则不易萌发;④15℃下形成的菌核易产生子囊盘,而其它温度下形成的菌核都不能萌发;⑤4种温度下形成的菌核都不能萌发。5类菌株各占2％、6％、22％、6％和64％。对第2、3类菌株而言,形成菌核时温度越高,菌核越易萌发。进一步分析说明菌核萌发多样性和菌株来源有一定的关系。对其中3个菌株的21个单子囊孢子后代菌核萌发特性的研究结果表明,菌核萌发特性具有遗传稳定性。这说明核盘菌菌株间菌核萌发习性存在着明显的多样性,且具有遗传稳定性,可用于研究这一病菌的群体结构。
The sclerotia of Sclerotinia sclerotiorum from different hosts and regions were produced at 15,20,25 and 28 C on PDA plates. Then, they were induced to germinate under 20℃. The results showed that 50 isolates could be divided into 5 types. (1) sclerotia could easily conduct myceliogenic germination; (2)sclerotia formed at all temperatures could germinate to apothecia; (3)sclerotia produced at 25 and 28 ℃ could germinate to apothecia, whereas those formed at 15 and 20 ℃ could not germinate; (4) sclerotia produced at 15℃ could germinate to apothecia, whereas those formed at other temperatures could not germinate; and (5)sclerotia produced at all temperatures could neither germinate to mycelia nor to apothecia. They took up 2%,6%, 22%,6% and 64%, respectively. The diversity was related to some extent with both geographic and host origins of the pathogen isolates.
Using the same method, 21 monoascospore cultures from 3 isolates representing 3 germination types were analysed for their sclerotial germinations, it indicated that the germination characteristics were genetically stable. So.it can be used to detect population diversity of the pathogen.
出处
《植物保护学报》
CAS
CSCD
北大核心
1997年第1期59-64,共6页
Journal of Plant Protection
基金
国家自然科学基金
湖北省自然科学基金
关键词
核盘菌
菌核萌发
多样性
Sclerotinia sclerotiorum , diversity, germination
