摘要
为了研究UVB对两栖类动物早期胚胎发育的影响,以中华大蟾蜍为研究对象,用UVB分别以不同强度和不同时间照射其二细胞期胚胎,观察记录胚胎的发育过程、统计死亡率并在形态学上对UVB照射诱导的畸胚进行分析。结果表明,30μW/cm2强度的UVB分别照射二细胞期胚胎20 s、60 s和80μW/cm2强度分别照射20 s、60 s、120 s后,对中华大蟾蜍早期胚胎发育有促进作用,使发育过程中的死亡率降低,畸胚出现的类型较少且畸变程度低;80μW/cm2强度的UVB照射二细胞期胚胎600 s,100μW/cm2强度的UVB照射20 s、60 s、600 s,120μW/cm2强度的UVB照射20 s,导致胚胎发育过程中死亡率明显升高,畸胚数量多且畸变程度高。结论:UVB照射剂量和时间不同,对中华大蟾蜍早期胚胎发育的影响有明显差异。
To study the effects of UVB on development of early stage embryos of amphibian, two-cell stage embryos of Bufo bufo gargarizans Cantor were irradiated by UVB with various intensity and time. The results showed that compared with control and other irradiated groups, when exposured to low intensity and short time, such as 30 μW/cm^2 in 20 s, 60 s and 80 μW/cm^2 in 20 s, 60 s, 120 s respectively, embryos development process was promoted, and embryos＇ death rate and degree of deformities were reduced. On the contrary, when irradiated by high intensity and long time, such as exposured to 80 μW/cm^2 in 600 s and 100 μW/cm^2 in 20 s, 60 s, 600 s and 120 μW/cm^2 in 20 s respectively, embryos＇ death rate increased. Then it could be concluded that various radiation intensity and time of UVB result in different effects on early stage embryos of Bufo bufo gargarizans Cantor.
出处
《四川动物》
CSCD
北大核心
2007年第2期297-301,I0006,共6页
Sichuan Journal of Zoology
基金
国家自然科学基金资助项目(30570199)
关键词
UVB
早期胚胎
中华大蟾蜍
畸胚
死亡率
UVB
early stage embryo
Bufo bufo gargarizans Cantor
abnormal embryo
death rate
