摘要
目的:探讨肝硬化门静脉高压症(portal hypertension,PHT)时局部血管紧张素原mRNA表达与核因子-kappa B(nuclear factor-kappa B,NF-κB)的活化及相关性在门静脉高压性血管病变中的意义。方法:采用化学发光凝胶电泳迁移率(chemiluminescent electrophoretic mobility shift assay)方法检测局部NF-κB的活性;以逆转录-聚合酶链反应方法检测肝硬化PHT病人脾脏动、静脉组织和正常血管局部血管紧张素原mRNA的表达情况。结果:对照组内脾脏动、静脉组织局部血管紧张素原mRNA分别为0.23±0.12、0.18±0.10,显著低于肝硬化PHT组脾动、静脉组织局部血管紧张素原mRNA的表达[0.48±0.21、0.43±0.16,P<0.05];对照组脾动、静脉局部NF-κB未检测到明显的活性,于肝硬化PHT组检测到具显著活性NF-κB的表达(P<0.05);肝硬化PHT组脾脏动、静脉血管紧张素原mRNA表达与NF-κB的活性呈显著正相关(P<0.05)。结论:肝硬化PHT病人局部血管紧张素原mRNA表达增强、NF-κB的活化可能是肝硬化PHT时内脏血管病变形成和发展的原因之一。
Objective To investigate the expression of local angiotensinogen mRNA and activation of local nuclear factor-κB in vasculopathy of portal hypertension （PHT） and to discuss their role in the pathogenesis of portal hypertensive vasculopathy. Methods The expression of local angiotensinogen mRNA in splenic artery and vein of PHT patients and normal vascular were detected by RT-PCR analysis. Chemiluminescent electrophoretic mobility shift assay was used to detect the activation of NF-κB in splenic artery and vein. Result Levels of local angiotensinogen mRNA in splenic artery and vein in PHT group were significantly higher than those in control group （0.48 ± 0.21 vs 0.23 ± 0.12 and 0.43 ± 0.16 vs 0.18 ± 0.10, both P 〈 0.05）. There was no significant difference of the expression of local angiotensinogen mRNA between the splenic artery and vein in PHT group. The activation of NF-κB in splenic artry and vein in PHT patients was significantly higher than that in control group （P 〈 0.05）. The expression of local angiotensinogen mRNA in splenic artery and vein in PHT patients had a significantly positive correlation with the activity of NF-κB （r=0.692, P 〈 0.05; r=0.703, P 〈 0.05, respectively）. Conclusion Local angiotensinogen and activation of NF-κB may be one of the pathogenic factors of portal hypertensive vasculopathy.
出处
《实用医学杂志》
CAS
2007年第10期1443-1446,共4页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:30170920)
