摘要
目的了解5/6肾切除大鼠残余肾组织核转录因子κappaB(NF-κB/IκBα)的表达与肾功能损害的关系,并观察温脾汤对NF-κB/IκBα的影响。方法采用5/6肾切除方法建立肾纤维化大鼠模型,以NF-κB p65亚基单抗及IκBα多抗为一抗,采用二步法免疫组化染色检测大鼠残余肾脏中NF-κB p65/IκBα的表达,结合血清肌酐、尿素氮、24 h蛋白尿等肾功能指标及肾脏病理损害情况,分析它们在肾脏损害中的作用,在此基础上阐明温脾汤肾保护作用与核转录因子-κB/IκB的关系。结果模型组肾组织中NF-κB p65的表达较正常组织显著增高,与尿蛋白及血肌酐增高呈正相关关系,而IκBα表达明显低于正常肾组织。与模型组相比,温脾汤可上调IκBα的表达,抑制NF-κB p65的过度活化。结论温脾汤能减轻5/6肾切除对大鼠肾功能的损伤,这一作用与其能降低肾组织NF-κB表达的异常增强有关。
Objective To understand the relationship between renal lesion and nuclear transcription factor-κB/IKBα （NF-κB/IKBα） expression in residual kidney of rat after nephrectomy by 5/6 and observe the influence of Wenpi Decoction on NF-κB/IKBα expression. Method The rat model of renal fibrosis was established by nephrectomy by 5/6. The NF-κB p65/IKBα expression in residual kidney was detected by using two - step stain of immunohistoehemistry and their actions in renal lesion were analyzed according to some indexes, such as levels of Scr, GUN and urine protein of 24 hours and renal pathological lesion degree. The relationship between the renal protective effect of Wenpi Decoction and NF-κB/IKB was explained on the base of above analysis. Result In the model group the NF-κB p65 expression in renal tissue was higher than that in normal renal tissue and correlated to the increase of urine protein and Scr, while IKBα expression was lower than that in normal renal tissue. By comparing with the model group Wenpi Decoction can up-regulate IKBα expression and inhibit the over activation of NF-κB p65. Conclusion Wenpi Decoction can relieve the renal lesion induced by nephrectomy by 5/6, which may be related to it can inhibit the abnormal increase of NF-κB expression in renal tissue.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2007年第4期239-241,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京市自然科学基金重点项目(No.7001001)
教育部长江学者和创新团队发展计划项目(No.IRT0413)
