摘要
目的:观察乙酰半胱氨酸(NAC)对不同病程糖尿病(DM)大鼠心肌缺血再灌注(I/R)后心功能的影响。方法:72只糖尿病大鼠随机分为假手术组(DS组,n=24)、I/R组(DI/R组,n=24)和I/R+NAC治疗组(DN组,n=24)。每组再随机分为2组(n=12),分别在2周(DS2、DI/R2和DN2)和8周(DS8、DI/R8和DN8)时进行实验。记录左心室发展压(LVDP)、收缩期和舒张期左心室内压的最大变化速率(±dp/dtmax),检测肌酸激酶同工酶(CK-MB)活性和丙二醛(MDA)含量。结果:(1)基础状态下,DS8和DI/R8组大鼠各项心功能参数低于对应的DS2和DI/R2组(P<0.05)。DN8组心功能参数高于DI/R8组,但低于DN2组(P<0.05)。(2)缺血/再灌注后,DI/R8组LVDP和±dp/dtmax%的恢复百分率低于DI/R2组和DN8组(P<0.01),CK-MB和MDA高于DI/R2组和DN8组(P<0.01);DN8组心功能参数恢复百分率低于DN2组(P<0.01)。结论:8周DM鼠基础心功能低于2周DM鼠,I/R后心功能受损程度比2周DM鼠严重,补充NAC可改善8周DM鼠基础心功能,但防治I/R损伤效果不如2周DM鼠。
OBJECTIVE To evaluate the effect of N-acetylcysteine on ischemia/reperfusion （I/R）-induced changes of cardiac function in different phases of diabetes mellitus（DM）. METHODS 72 diabetic rats induced by streptozotocin（STZ） were randomly seperated into 3 groups： sham operation group（Ds, n = 24）, diabetic I/R group（DI/R, n = 24）, diabetic rats group treated with NAC（DN,n = 24） . Following 2 weeks （DS2, DI/R2 and DN2, n = 12） and 8 weeks（DS8, EI/R8 and DN8, n = 12） ,experiments were carried out. 1 week after the experimental diabetes induced, animals in DN group were treated with NAC 100 mg·kg^-1 by intragastric administration（two times per day）. Left ventricular developed pressure（LVDP）and dp/dtmax were measured and recorded using computerized data acquisition system throughout the experiment. Creatine kinase isoenzyme-MB（CK-MB） and myocardial malondialdehyde （MDA） were measured. RESULTS （1） Under basal conditions, the values of HR, LVDP, and dp/dmax were lower in DS8 and DI/R8 group than in DS2 and DI/R2 group respectively, and the above indices were higher in DN8 group than in DI/R8 group but lower than in DN2 group. （2） The recovery of cardiac function was better in DN8 group than in DI/R8 group but worse than in DN2 group after I/R, and the cardiac function recovered better in DI/R2 group than in DI/R8 group. CONCLUSION The hearts from 8 weeks diabetic rats are more susceptible to I/R induced cardiac dysfunction than those from 2 weeks diabetic rats, and NAC can not only improve the decreased basic cardiac function but also attenuate cardiac dysfunction induced by I/R, but the effects are less effective in 8 weeks diabetic rats than in 2 weeks diabetic rats.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第5期590-592,共3页
Chinese Journal of Hospital Pharmacy
基金
教育部留学回国人员科研启动基金[编号:教外司留(2003)14号]
