摘要
克隆并测定了四川黑熊(Ursus thibetanus mupinensis)线粒体基因组764 bp的片段,根据序列同源性比较,该DNA片段包括2个蛋白质编码基因:ND3和ND4L基因,以及1个tRNA-Arg基因.四川黑熊的ND3基因和ND4L基因的DNA序列和所编码的氨基酸序列与马来熊、棕熊、美洲黑熊和北极熊的同源性分别为:90%和95%、93%和97%、91%和96%、93%和97%;89%和98.98%、88%和98.98%、89%和100%、89%和98.98%.与棕熊、美洲黑熊和北极熊tRNA-Arg(CGA)的同源性分别为95.65%、94.20%和94.20%.拓扑结构比较显示,四川黑熊的ND3基因所编码的氨基酸序列比美洲黑熊的少了1个蛋白激酶C磷酸化位点.
The fragment of mitochondrial genome 764 bp of Sichuan black bear has been cloned and determined. According to homologous comparision, the DNA fragment contains two protein-coding genes （ND3 and NIML） and one tRNA-Arg gene. The DNA sequence with amino acid sequence of black bear＇s ND3 and NIML gene and the DNA sequence with amino acid sequence of Malay bear＇ , brown bear＇ , American black bear＇ and polar bear＇ ND3 and NIML gene have the high homology, the homology is 90% and 95%, 93% and 97%, 91% and 96%, 93% and 97%, respeclively; 89% and 98.98%, 88% and 98.98%, 89% and 100%, 89% and 98.98%, respeclively. The homology of tRNA-Arg gene with brown bear,American black bear and polar bear＇ is 95.65%, 94.20% and 94.20% , respeclively. The analysis of topology indicates that ND3 gene of Asian black bear has a less Protein kinase C phosphorylation site than American black bear＇ s.
出处
《四川师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第3期398-401,共4页
Journal of Sichuan Normal University（Natural Science）
基金
国家自然科学基金(30570275)
四川省应用技术基金
四川省教育厅自然科学重点基金
四川省重点学科建设基金资助项目
关键词
四川黑熊
线粒体基因组
基因克隆
序列分析
Asian black bear Sichuan subspecies （ Ursus thibetanus mupinensis）
Mitochondrial genome
Gene cloning
Sequence analysis
