摘要
目的:研究新型钛铌锆锡合金(Ti-24Nb-4Zr-7.9Sn)的表面能大小以及对成骨细胞在其表面的附着及骨架蛋白actin、vinculin表达的影响。方法:以纯钛为对照,采用表面接触角测量的方法对合金的表面能进行研究。将原代培养的成骨细胞接种到合金表面,观察第30 min,60 min,120 min细胞在合金表面的附着情况,采用荧光显微镜观察骨架蛋白actin、vinculin在合金表面的表达。结果:表面能测试结果显示合金的表面能(67.40 mJ/m2)略高于纯钛的表面能(62.43 mJ/m2);成骨细胞在合金表面的附着情况与对照组间无显著性差异,骨架蛋白actin、vinculin染色显示细胞在合金表面伸展良好。结论:成骨细胞在新型钛铌锆锡合金表面附着良好,其和成骨细胞在纯钛表面的附着未见显著性差异。
AIM： To evaluate the surface energy and the affection of a new Ti - 24Nb - 4Zr - 7.9Sn alloy on the cell behavior , including cell adhesion kinetics and cytoskeletal organization. METHODS ： The surface energy was examined by physicochemical methods. Primary osteoblast culture was established from rat calvaria. Cell adherence on the alloy was examined at 30 min, 60 min and 120 min. The expression of actin and vinculine was observed by fluores- cence microscope. RESULTS： The new alloy has higher surface energy than pure titanium. Throughout the 120 min period of study, adhesion of the osteoblast to the new alloy was similar to that of the pure titanium. Good cell spreading was shown by actin and vinculin immunostaining. CONCLUSION： The new Ti -24Nb-4Zr- 7.9Sn alloy possesses higher surface energy than pure titanium, adhesion of osteoblast to the new alloy is similar to that to pure titanium.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第5期255-257,共3页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金(50471074
30471754)
第四军医大学后备人才基金资助
关键词
钛合金
成骨细胞
纯钛
附着
Ti alloy
osteoblast
pure Ti
adhension
