摘要
目的研制T-2毒素标准品的替代品并应用于酶联免疫吸附试验(ELISA)。方法在研制了抗T-2毒素单抗的基础上,将抗体用无花果蛋白酶进行酶切,制备抗T-2毒素单抗F(ab)2片段,并以F(ab)2片段作为免疫原免疫日本大耳白兔,研制出抗T-2毒素单抗独特型抗体。结果所研制的抗独特型抗体经过鉴定,是Ab2β型,与T-2毒素存在内影像关系。结论该抗体可以替代T-2毒素标准品应用于ELISA检测。
Objective To explore a substitute for standard T-2 toxin and apply it to enzyme-linked immunosorbent assay （ ELISA）. Method The polyclonal anti-idiotype antibody against anti-T-2 toxin monoclonal antibody was prepared by immunizing thd Japanese flap-eared rabbit with anti-T-2 toxin monoclonal antibody＇s F （ab＇）2. Results The obtained polyclonal anti- idiotype antibody against anti-T-2 toxin monoclonal antibody was identified as Ab2β subclass. Conclusion The obtained polyclonal anti-idiotype antibody was the ＂inner image＂ with T-2 toxin and can be used to replace T-2 toxin standard in ELISA.
出处
《中国食品卫生杂志》
2007年第3期234-237,共4页
Chinese Journal of Food Hygiene
关键词
T-2毒素
抗体
抗独特型
酶联免疫吸附试验
T-2 Toxin
Antibody, Anti-Idiotypic
Enzyme-Linked Immunosorbent Assay
