摘要
目的建立用HPLC法测定异烟肼注射液含量的方法。方法HPLC法，采用Hypersil BDS C18柱，以乙腈-0．075mol·L^-1磷酸二氢钠溶液（体积比5：95）为流动相，流速为1．0mL·min^-1，检测波长262nm。结果异烟肼在9．90～99．00μg·mL^-1浓度范围内与峰面积线性关系良好（r=0．9999），平均回收率100．3％（RSD=1．0％）。结论本法操作简便，快速准确，重复性好，可作为本品质量控制方法。
Objective To establish an HPLC method for determining the content of isoniazid in isoniazid injection. Method Samples were analyzed on an Hypersil BDS C18 column using acetonitrile-0.075 mol · L^-1 sodium dihydrogen phosphate （5：95 ） as mobile phase. The flow rate was 1.0 mL · min^ -1 and the detection wavelength 262 nm. Results There was a good linearity for isoniazid in the range of 9.90 -99.00 μg · mL^-1（ r = 0.9999）. The average recovery was 100.3% with RSD 1.0%. Conclusion The method is simple, rapid and accurate, which is suitable for the quality control of isoniazid injection.
出处
《广东药学院学报》
CAS
2007年第2期157-158,共2页
Academic Journal of Guangdong College of Pharmacy
