摘要
目的建立人结肠癌鸡胚移植模型，研究促血管生成素-2（Ang-2）抗体对其血管生成的影响。方法将人结肠腺癌细胞系HT-29细胞接种到鸡胚绒毛尿囊膜（CAM）上，通过解剖显微镜、光镜及免疫组织化学方法，动态观察移植瘤的血管生成特性及Ang-2抗体对其血管生成的影响。结果HT-29细胞系接种到CAM第3-7天，瘤体迅速生长，大量血管呈放射状向瘤体集中．长入或跨越接种区．部分大血管向瘤体弯曲并相应扩张，形成血管辐辏现象。给予Ang-2抗体后第5天在解剖显微镜下进行血管计数，Ang-2抗体组的血管数（37．2±4．6）明显低于癌细胞对照组（56．8±7．4），但高于空白对照组（29．5±3．1），各组间差异有统计学意义（P〈0．01）。组织学检查Ang-2抗体组的微血管密度（9．6±2．4）明显低于癌细胞对照组（20．2±5．8），两者差异有统计学意义（P〈0．01）。结论Ang-2抗体能抑制人结肠腺癌细胞系HT-29细胞诱导的血管生成，为结肠癌的抗血管生成治疗提供了新的途径。
Objective To establish an implanted model of human colonic carcinoma on chick embryo, and to study the effects of anti-angiopoietin-2 antibody on its vascularization. Methods The human colonic adenocarcinoma cell line HT-29 was transplanted on the chick embryo＇s chorioallantoic membrane （CAM）, and the angiogenesis characteristics were observed by stem-microscope, light microscope and immunohistochemistry. Furthermore, the effects of anti-angiopoietin-2 antibody on angiogenesis and tumor growth were also investigated. Results Three to seven days after HT-29 cell line was implanted into CAM, tumors grew rapidly and new blood vessels grew toward tumors. Five days after anti-angiopoietin-2 antibody was given, the number of blood vessels in anti-angiopoietin-2 antibody group was significantly down-regulated than that in tumor control group observed by stero- microscope（37.2±4.6 vs 56.8±7.4,P 〈 0.01 ）, but was up-regulated than that in normal control group （37.2±4.6 vs 9.6±2.4,P 〈 0.01）. Microvessel density（MVD） in anti-angiopoietin-2 antibody group was much lower than that in tumor control group by histological examination （9.6±2.4 vs 20.2±5.8,P 〈 0.01）. Conclusion Angiopoietin-2 antibody is able to inhibit the angiogenesis induced by colorectal cancer cell line HT-29 obviously. The anti-angiopoietin-2 antibody may be potentially useful for clinical treatment of colonic carcinoma.
出处
《中华胃肠外科杂志》
CAS
2007年第3期278-280,共3页
Chinese Journal of Gastrointestinal Surgery
