摘要
目的 建立一种高效液相色谱法测定复方川脊片中芍药苷的含量.方法 采用岛津LC-10AT高效液相色谱仪,选用Symmetry-C18分析柱（5 μm ,3.9 mm×150 mm）为固定相,以异丙醇-甲醇-醋酸-水（1：23：1：75）为流动相;流速：1.0 mL·min^-1;柱温为35 ℃;检测波长为230 nm.结果 复方川脊片中芍药苷与其他组分良好分离.芍药苷进样量线性范围在0.204 8～1.024 0 μg,r=0.999 3;样品加样回收率为98.6%（n＝6）, RSD=0.835%（n＝6）.结论 该含量测定方法简便,准确,重复性好,可用于控制复方川脊片的质量.
Objective To develop a HPLC method for the determination of the content of paeoniflorin infufang chuanji tablets. Methods Paeoniflofin was separated on a Symmetry-C18 column（5 μm ,3.9 mm×150 mm ） with the use of isopropanol-methanol-acetic acid-water（1 ： 23 ： 1 ： 75） as the mobile phase. The flow rate was 1.0 mL·min^-1, the column temperature was 35 ℃ and UV detection wavelength , 230 nm. Results Paeoniflorin was well separated from the other comporents of the tablets. The linear range of concentration of paeoniflorin was 0. 204 8 - 1. 024 0 μg, the correlation coefficient r = 0.999 3, the average rate of recovery was 98.6% （ n = 6 ） , RSD = 0. 835%, （ n = 6 ）. Conclusion The method was shown to he handy , accurate and well reproducible in the content determination of paeoniflorin infufang chuanji tablets.
出处
《医药导报》
CAS
2007年第6期665-666,共2页
Herald of Medicine
关键词
川脊片
复方
芍药苷
含量测定
色谱法
高压液相
Fufang chuanji tablets
Paeoniflorin
Content determination
Chromatography, high pressure liquid
