摘要
对芦笋的启动培养、试管繁殖及其移栽技术进行了研究，建立了组培快繁技术体系。结果如下：以出土嫩茎绿色部份为外植体。MS＋NAA0．1mg／L＋BA0．1mg／L为启动培养基．萌芽率可达53．7％；以MS培养基附加NAA0.1mg／L＋KT01mg／L＋BA0．3～0．4mg／L，茎基和茎中段为材料的增殖系数分别达5．40～6．41、3．41～5．19；茎尖在1／2MS＋KT0.1mg／L＋1AA0.5mg／L ＋NAA0.1mg ／L＋IBA2．0mg／L生根培养24d，之后，在不加任何激素的MS培养基上培养26d，根长达4.63～5.05cm，根数10．9～12．1条。移栽时以椰糠作为基质，成活率达95．20％。
The technological systems of tissue culture for Asparagus＇rapid propagation was established through the study about its bud inhation and rapid propagation and transplantation . MS mediume supplemented with 0.1 mg/L NAA ＋ 0. 1 mg/L BA was the initiative medium,with a survival rate of 53.7 % for green shoots. MS supplemented with 0.1 mg/L NAA ＋ 0. lmg/L KT ＋ 0.3 -0.4 mg/L BA,the multiplication coefficient being 5.40 - 6.41 and 3.41 - 5.19 for stem-base and middle stem. 1/2MS supplemented with 0.1 mg/L KT ＋ 0.5 mg/L IAA ＋ 0.1 mg/L NAA ＋2.0mg/L IBA was the rooting medium of shoot tips for 15 days,and the average root length of 4.63 -5.05 cm, root number of 10.9-12.1 after 20 days＇ culture with MS medium without any plant hormones. The transplantation survival rate was 95.20 % with medium of coir dust.
出处
《西南农业学报》
CSCD
2007年第3期470-473,共4页
Southwest China Journal of Agricultural Sciences
基金
福建省科技攻关项目"芦笋新品种引进及全雄系工厂化育苗技术的开发与应用"(2002N033)
关键词
芦笋
组织培养
快速繁殖
移裁
Asparagus offcinalis L.
tissue culture
rapid propagation
transplantation
