摘要
目的研究经结构修饰后的异黄酮类化合物F11是否具较好的体外、体内抗癌活性。方法采用MTT法,以5,7,4′-三羟基异黄酮(genistein,GEN)作为对照,研究化合物F11对Hela细胞的抗增殖作用;采用荷瘤裸鼠模型,以GEN和环磷酰胺作为对照,研究化合物F11在体抗癌效应。结果MTT实验结果显示,在相同的作用浓度下,F11处理组Hela细胞形态异常改变,死细胞明显增多,细胞存活率显著低于GEN作用组(P<0.05)。荷瘤动物实验结果表明与GEN相比,F11对Hela细胞裸鼠移植瘤的生长有显著的抑制作用(P<0.01),效果与环磷酰胺相当,且未观察到毒副反应。结论异黄酮类化合物经过特定结构修饰,可显著增强其抗癌效应,有值得进一步研究开发的潜在价值。
Objective To investigate whether structurally modified isoflavone derivatives have better antitumor activity in vivo and in vitro. Methods The antiproliferative activity of compound F11 against Hela strain cells was analyzed by MTT and compared with that of Genistein. The nude mouse loading Hela tumor cells was used to evaluate the in vivo antitumor activity of F11 and the results were compared with that of Genistein and Cytoxan. Results Compound F11 had better performance than Genistein in antitumor tests as revealed by in vitro MTT measurement and in vivo nude mice model loading Hela tumor cells. Conclusion Isoflavone derivatives could be modified to acquire potent eytotoxieity on tumor cells, which are of potential clinical value and worth further investigation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第12期1155-1157,共3页
Journal of Third Military Medical University
基金
重庆市院士基金(2004BC5006)~~
